水产学报2013，Vol.37Issue(6)：830-839,10.DOI:10.3724/SP.J.1231.2013.38463
杂色鲍同种移植炎症因子1的克隆及其在应激下的表达
Molecular cloning and expression of allograft inflammatory factor 1 in Haliotis diversicolor under stresses
摘要
Abstract
Allograft inflammatory factor-1 (AIF-1) is a kind of calcium-binding protein with EF-hand domain.AIF-1 mRNA is robustly induced by IFN-γ in murine macrophages.It is involved in transplant rejection,immune inflammatory reaction,non-inflammatory injury and so on.In this study,a molluscan AIF-1 gene,HdAIF-1,was cloned for the first time from Haliotis diversicolor.Its full-length cDNA sequence is 942 bp,with a 456 bp open reading frame encoding a protein of 151 aa.Quantitative real-time PCR results indicated that HdAIF-1 could be detected in all examined tissues,with the highest level in hemolymph and gill.Under thermal stress,HdAIF-1 was up-regulated in gill significantly at temperature-rise period and achieved the highest level when up to 31 ℃.However,the expression level of HdAIF-1 in hemolymph and hepatopancreas did not show significant difference between thermal group and control group from the first phase to the fourth,and its expression level was up-regulated significantly at 96 h in these two tissues.Under hypoxia stress,the expression level of HdAIF-1 in hemolymph showed no significant difference between control and exposed groups.However,it was down-regulated at 24 h and up-regulated at 192 h remarkably in gill.After Vibrio parahaemolyticus challenge,the HdAIF-1 expression level achieved the highest level at 3 h in hemolymph,reduced to the control level at 12 h,and then up-regulated significantly at both 24 h and 48 h.These results suggest that HdAIF-1 may play important roles as an immune factor under different stresses.关键词
杂色鲍/同种移植炎症因子/高温/缺氧/副溶血弧菌Key words
Haliotis diversicolor/allograft inflammatory factor 1 (AIF-1)/thermal/hypoxia/Vibrio parahaemolyticus分类
生物科学引用本文复制引用
黄贻涛,蔡秀红,张子平,王国栋,邹志华,王淑红,王艺磊..杂色鲍同种移植炎症因子1的克隆及其在应激下的表达[J].水产学报,2013,37(6):830-839,10.基金项目
国家自然科学基金项目(41006105 （）
41176152) （）
集美大学创新团队基金(2010A001) （2010A001）
