四川中医2013，Vol.31Issue(6)：60-63,4.
基因芯片技术阐释虚寒证与虚热证大鼠“精气夺则虚”的分子机制
Explanation on the Molecular Mechanism of "Lack of Vital Essence Resulting in Deficiency Syndrome" of Asthenia Cold Syndrome and Asthenia Hot Syndrome Rats
摘要
Abstract
Objective:To explain the molecular mechanism of "lack of vital essence resulting in deficiency syndrome" in both asthenia cold syndrome rats and asthenia hot syndrome rats by gene chip technology.Methods:The Asthenia cold and hot syndrome rats models were induced by compound preparation of Traditional Chinese Medicine.The liver gene expression in each group was detected by gene chip.We selected the differential expression genes,analyzed the genes by cluster and conducted the significant analysis on the genetic function of differential genes.A part of genes were selected to test the accuracy of results by RT -PCR.Results:The cluster analysis showed the profile of genes expression of asthenia cold model group was similar to asthenia hot model group.Both asthenia cold model group and asthenia hot model group were different from control group.Among asthenia cold model group,asthenia hot model group and control group,there were 28 strips of differential expression gene,among which 16 stips were about immune response.Conclusion:Obvious changes have been both arose in asthenia cold model group and asthenia hot model group about genes immune response,which is possibly the molecular mechanism of "lack of vital essence resulting in deficiency syndrome".关键词
基因芯片/虚寒证/虚热证/精气夺则虚Key words
Gene chip/ Asthenia cold syndrome/ Asthenia hot syndrome/ Lack of vital essence resulting in deficiency syndrome分类
医药卫生引用本文复制引用
韩冰冰,王世军..基因芯片技术阐释虚寒证与虚热证大鼠“精气夺则虚”的分子机制[J].四川中医,2013,31(6):60-63,4.基金项目
国家重点基础研究发展计划(973计划)课题资助(编号:2007CB512601,基于四性的中药性-效-物质关系研究),科技部973项目子课题(编号:2013CB531803) （973计划）
