山东农业科学2013，Vol.45Issue(6)：4-10,7.
水稻ERECTA基因组DNA的克隆及植物表达载体构建
Cloning of Rice ERECTA Genomic DNA and Construction of Plant Expression Vector
摘要
Abstract
Based on the reported OsERECTA cDNA sequence in rice (GenBank accession number:Os06g0203800),its corresponding genomic DNA sequence (gERECTA) was researched by the Blast tool of NCBI.Two pairs of primers were designed to isolate the upstream and downstream segments separately,and then the two segments were joined together to form an intact gERECTA gene and subcloned into an expression vector pCAMBIA1390.The full length of the cloned gERECTA was 8 901 bp,including 1.7 kb of 5'and 300 bp of 3'regions.The recombinant plasmid pCAMBIA1390-gERECTA was identified by double digestion and sequencing,which suggested that the plant expression vector pCAMBIA1390-gERECTA was successfully constructed.关键词
水稻/OsERECTA基因/克隆/植物表达载体Key words
Rice/ OsERECTA gene/ Cloning/ Plant expression vector分类
生物科学引用本文复制引用
韩同凯,王盈盈,林红珍,陈翠霞,谢先芝..水稻ERECTA基因组DNA的克隆及植物表达载体构建[J].山东农业科学,2013,45(6):4-10,7.基金项目
国家自然科学基金(30870192)和山东省自然科学杰出青年基金(2009JQ11)资助. （30870192）
