山东医药2013，Vol.53Issue(17)：1-3,6,4.DOI:10.3969/j.issn.1002-266X.2013.17.001
Th17及Th2细胞在博莱霉素致小鼠肺纤维化中的表达及相互关系
Expression and interrelation of Th17 and Th2 cells in a murine model of bleomycin-induced pulmonary fibrosis
摘要
Abstract
Objective To investigate the expression and interrelation of Th17 cells and Th2 cells in a murine model of bleomycin-induced pulmonary fibrosis.Methods A total of 20 female BALB/c mice were randomly divided into two groups (10 in each group):the bleomycin group (BLM group) and control group.The BLM group was daily injected with BLM to establish PF model,the control group was injected with PBS,and both of the two groups were continuously injected for four weeks.HE and Masson staining were used to detect the pulmonary pathological changes of two groups and to evaluate the fibrosis score.The alkali hydrolysis was used to detect the content of hydroxyproline (HYP) in lung tissue.RTPCR was used to detect the mRNA expressions of IL-6 of lung.Enzyme linked immunosorbent assay (ELISA) was used to measure the contents of IL-17A,IL-6 in peripheral blood,and the levels of Th17 cells and Th2 cells in peripheral blood and lung were determined by using the flow cytometry.Then the correlation analysis between all the above parameters was conducted.Results Compared with control group,the lung HYP,the score of PF,the IL-17A,IL-6,Th17 cells,Th2 cells of BLM group were obviously increased (all P ＜0.01).The Th17 cells,Th2 cells,and the contents of IL-17A and IL-6 in peripheral blood respectively had a positive correlation with pulmonary fibrosis and HYP contents of lung.The Th17 cells,Th2 cells,and the levels of IL-6mRNA in lung respectively had a positive correlation with pulmonary fibrosis and HYP contents of lung.The Th17cells of lung respectively had a positive correlation with Th2 cells,IL-6 and IL-17A.Th2 cells of lung respectively had a positive correlation with IL-17A and IL-6.Conclusions Th17 and Th2 cells are closely related to PF,both of which may be involved in the pathogenesis of PF,and have a synergistic effect.IL-6 may be the key factor of affecting the interaction between Th17 and Th2 cells.关键词
肺纤维化/博来霉素/Ⅱ型辅助性T细胞/T辅助细胞17/白细胞介素/小鼠Key words
pulmonary fibrosis/bleomycin/type Ⅱ helper T cells/T helper 17 cells/interleukin/mice分类
医药卫生引用本文复制引用
谢佳峻,钟小宁,雷玲,李宇..Th17及Th2细胞在博莱霉素致小鼠肺纤维化中的表达及相互关系[J].山东医药,2013,53(17):1-3,6,4.基金项目
国家自然科学基金资助项目(81160010) （81160010）
广西自然科学基金资助项目(2011GXNSFA018178). （2011GXNSFA018178）
