山东医药2013，Vol.53Issue(17)：15-17,3.DOI:10.3969/j.issn.1002-266X.2013.17.006
线粒体ATPase-6基因多态性与男性精子活力的关系
Association between mitochondrial ATPase-6 gene polymorphism with male sperm motility
摘要
Abstract
Objective To investigate the association between mitochondrial ATPase-6 gene polymorphism with male sperm motility.Methods There were 319 cases of patients with asthenozoospermia (observation group) and 344 cases of normal sperm mobility people (control group).The mitochondrial ATPase-6 gene (+9 052 bp) Hae Ⅱ restriction enzyme cutting site polymorphism was detected by PCR-RFLP analysis technique.Then the genotype distribution and allele frequency of each group were detected by the Hardy-Weinberg equilibrium test,and the association between ATPase-6 gene mutation frequency and sperm motility was analyzed.Results The hh,Hh and HH genotype distributions in observation group were 19.74％,1.25％ and 79.01％,while they were 4.94％,0.58％ and 94.45％ in control group,both the genotype frequency distributions were within the range of Hardy-Weinberg equilibrium; but the mutant gene (h) frequency in observation group was significantly higher than that in control group (P ＜ 0.01).Conclusion ATPase-6 (+ 9 052 bp)gene Hae Ⅱ restriction enzyme cutting site mutation may affect the efficiency of ATPase-6 translation,thereby affecting sperm motility and causing asthenozoospermia.关键词
弱精子症/精子活力/ATPase-6基因/ATP合成酶6亚基/聚合酶链式反应—限制性片段长度多态性分析Key words
asthenozoospermia/sperm motility/ATPase-6 gene/ATP synthase F0 subunit 6/polymerase chain reaction-restriction fragment length polymorphism分类
医药卫生引用本文复制引用
莫毅,梁方方,谢伟,王娟,叶健,谢丹尼..线粒体ATPase-6基因多态性与男性精子活力的关系[J].山东医药,2013,53(17):15-17,3.基金项目
广西壮族自治区人口和计划生育委员会科研课题(桂人口计生研1106). （桂人口计生研1106）
