山东医药2013，Vol.53Issue(20)：25-28,后插2,5.DOI:10.3969/j.issn.1002-266X.2013.20.008
外源性硫化氢对子宫肌瘤细胞增殖和凋亡的影响
Effect of exogenous hydrogen sulfide on the proliferation and apoptosis of human uterine leiomyoma cells
摘要
Abstract
Objective To observe the effect of exogenous hydrogen sulfide on the proliferation and apoptosis of human uterine leiomyoma cells and to investigate its mechanism.Methods The human uterine leiomyoma cells were randomly divided into the control and observation group.The cells were treated with different concentrations of sodium bisulfide (NaHS) (10-5 mol/L,10-4 mol/L and 10-3 tmoL/L) for 12,24 and 48 hours in the observation group.MTT assay was used to detect the cell proliferation inhibition rate.The apoptosis rates were measured by flow cytometry.The mRNA and protein expressions of p53 and Bcl-2 in human leimyoma cells were measured by Real-time PCR and Western blot.Results With the increase of NaHS concentration and the prolonged time,the cell proliferation inhibition rates were significantly increased (all P ＜ 0.05) and the apoptosis rates were significantly increased (all P ＜ 0.05) in human uterine leiomyoma cells.In the observation group,with the increase of NaHS concentration and the prolonged time,the mRNA and protein expressions of p53 were up-regulated (all P ＜0.05),and the mRNA and protein expressions of Bel-2 were down-regulated (all P ＜ 0.05).Conclusions Exogenous hydrogen sulfide inhibits the cell proliferation and promotes the apoptosis of human uterine leiomyoma cells,whose mechanism may be related to the up-regulation of p53 expression and down-regulation of Bcl-2 expression induced by hydrogen sulfide.关键词
硫化氢/细胞凋亡/细胞增殖/子宫肌瘤细胞/p53/Bcl-2Key words
hydrogen sulfide/apoptosis/cell proliferation/ uterine leiomyoma cells/p53/Bcl-2分类
医药卫生引用本文复制引用
唐彩霞,张树友,何英新,李兴,周寿红..外源性硫化氢对子宫肌瘤细胞增殖和凋亡的影响[J].山东医药,2013,53(20):25-28,后插2,5.基金项目
湖南省衡阳市科学技术发展计划项目(2012ks36). （2012ks36）
