山东医药2013，Vol.53Issue(21)：4-6,3.DOI:10.3969/j.issn.1002-266X.2013.21.002
不同浓度肿瘤坏死因子-α对肺腺癌A549细胞的影响
Effects of different concentrations of tumor necrosis factor-α on A549 cells
摘要
Abstract
Objective To observe the effects of different concentrations of TNF-α on the protection,inflammation and apoptosis of A549 cells.Methods A549 cells were treated with different concentrations of TNF-α (0,1,10,25 and 50 ng/mL) for six hours respectively.The cell viability was observed by MTT assay.Reactive oxygen species (ROS) levels were detected by applying ROS fluorescence probe DCFH-DA.The gene expressions of Nrf-2 and NF-κB were detected by RT-PCR.The cells were treated by nuclear fluorescence staining DAPI,and the apoptosis was observed by fluorescence microscope.Results TNF-α at the concentration of 0,1,10,25 and 50 ng/mL had no effect on A549 cell viability (all P＞0.05),and compared with the control group,the cell viability was decreased at the concentration of 100 ng/mL(P ＜0.05).With the increase of TNF-α concentration,the generation of ROS increased(all P ＜0.05),the mRNA expression of Nrf-2 decreased(all P ＜ 0.05),the mRNA expression of NF-κB increased(all P ＜ 0.05),and the apoptosis rate was on the rise.Conclusions Different concentrations of TNF-α can make A549 cells to produce different amounts of ROS and cellular effects.With the increase of TNF-α concentration,the cell damage is aggravated.Its mechanism may be related to the increasing amount of ROS induced by the increasing TNF-α concentration.关键词
肺肿瘤/肿瘤坏死因子/活性氧/细胞保护/炎症/细胞凋亡Key words
lung neoplasms/ tumor necrosis factor/ reactive oxygen species/ cytoprotection/ inflammation/ apoptosis分类
医药卫生引用本文复制引用
徐采云,李理,袁伟锋,黄文杰..不同浓度肿瘤坏死因子-α对肺腺癌A549细胞的影响[J].山东医药,2013,53(21):4-6,3.基金项目
国家自然科学基金资助项目(81070003). （81070003）
