山东医药2013,Vol.53Issue(21):14-16,3.DOI:10.3969/j.issn.1002-266X.2013.21.005
四种海螺中左卡尼汀及其酰化物含量比较
Determination of L-carnitine and its acylates in four sea snails
摘要
Abstract
Objective To detect the contents of L-carnitine (LC),acetyl-L-carnitine (ALC) and propionyl-L-carnitine (PLC) in four sea snails (Natica tigrina,Thais clavigera Kuster,Busycon canaliculatu,Cerithidea obtusa Lamarck).Methods The samples were homogenated and extracted by 3% perchloric acid,and derivatized with 1-AA (1-aminoanthracene),and then the contents of LC,ALC and PLC in four sea snails were detected by HPLC-FL with precolumn derivatization.Result The contents of LC,ALC and PLC were (39.67 ± 11.80) μmol/100 g,(7.91 ± 1.85) μmol/100 g and (2.26 ±0.66) μmol/100 g respectively in Natica tigrina; (44.02 ± 12.30) μmoL/100 g,(9.64 ± 1.56) μ mol/100 g and (0.71 ±0.13) μmol/100 g in Thais clavigera Kuster; (9.20 ± 1.51) μmol/100 g,(3.55 ± 1.03) μmol/100 g and (1.61 ±0.50) μmol/100 g in Busycon canaliculatu; and the contents of LC,ALC and PLC in Cerithidea obtusa Lamarck were (162.51 ± 34.09) μmol/100 g,(33.47 ± 13.41) μmol/100 g and (11.03 ± 1.84) μmol/100 g respectively.Conclusion LC,ALC and PLC were all detected in four sea snails,and the contents of LC,ALC and PLC were the highest in Cerithidea obtusa Lamarck.关键词
左卡尼汀/乙酰左卡尼汀/丙酰左卡尼汀/高效液相色谱法/螺Key words
L-carnitine/ acetyl-L-carnitine/ propionyl-L-carnitine/ high performance liquid chromatography/ snails分类
医药卫生引用本文复制引用
曹玉,王晨静,赵霞,李存友,王宏广,王春波,管华诗..四种海螺中左卡尼汀及其酰化物含量比较[J].山东医药,2013,53(21):14-16,3.基金项目
国家科技支撑计划(2008BAI54B02) (2008BAI54B02)
青岛市科技计划基础研究项目[11-2-4-2-(3)-jch]. (3)