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四种海螺中左卡尼汀及其酰化物含量比较

曹玉 王晨静 赵霞 李存友 王宏广 王春波 管华诗

山东医药2013,Vol.53Issue(21):14-16,3.
山东医药2013,Vol.53Issue(21):14-16,3.DOI:10.3969/j.issn.1002-266X.2013.21.005

四种海螺中左卡尼汀及其酰化物含量比较

Determination of L-carnitine and its acylates in four sea snails

曹玉 1王晨静 2赵霞 2李存友 3王宏广 3王春波 3管华诗3

作者信息

  • 1. 中国海洋大学医药学院,山东青岛266100
  • 2. 青岛大学医学院附属医院
  • 3. 青岛大学医学院
  • 折叠

摘要

Abstract

Objective To detect the contents of L-carnitine (LC),acetyl-L-carnitine (ALC) and propionyl-L-carnitine (PLC) in four sea snails (Natica tigrina,Thais clavigera Kuster,Busycon canaliculatu,Cerithidea obtusa Lamarck).Methods The samples were homogenated and extracted by 3% perchloric acid,and derivatized with 1-AA (1-aminoanthracene),and then the contents of LC,ALC and PLC in four sea snails were detected by HPLC-FL with precolumn derivatization.Result The contents of LC,ALC and PLC were (39.67 ± 11.80) μmol/100 g,(7.91 ± 1.85) μmol/100 g and (2.26 ±0.66) μmol/100 g respectively in Natica tigrina; (44.02 ± 12.30) μmoL/100 g,(9.64 ± 1.56) μ mol/100 g and (0.71 ±0.13) μmol/100 g in Thais clavigera Kuster; (9.20 ± 1.51) μmol/100 g,(3.55 ± 1.03) μmol/100 g and (1.61 ±0.50) μmol/100 g in Busycon canaliculatu; and the contents of LC,ALC and PLC in Cerithidea obtusa Lamarck were (162.51 ± 34.09) μmol/100 g,(33.47 ± 13.41) μmol/100 g and (11.03 ± 1.84) μmol/100 g respectively.Conclusion LC,ALC and PLC were all detected in four sea snails,and the contents of LC,ALC and PLC were the highest in Cerithidea obtusa Lamarck.

关键词

左卡尼汀/乙酰左卡尼汀/丙酰左卡尼汀/高效液相色谱法/

Key words

L-carnitine/ acetyl-L-carnitine/ propionyl-L-carnitine/ high performance liquid chromatography/ snails

分类

医药卫生

引用本文复制引用

曹玉,王晨静,赵霞,李存友,王宏广,王春波,管华诗..四种海螺中左卡尼汀及其酰化物含量比较[J].山东医药,2013,53(21):14-16,3.

基金项目

国家科技支撑计划(2008BAI54B02) (2008BAI54B02)

青岛市科技计划基础研究项目[11-2-4-2-(3)-jch]. (3)

山东医药

OACSTPCD

1002-266X

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