山东医药2013，Vol.53Issue(22)：13-15,21,4.DOI:10.3969/j.issn.1002-266X.2013.22.005
Nogo-A蛋白在大鼠心肌细胞中的表达变化及其在细胞缺氧性凋亡中的作用
Expression of Nogo-A protein in rat cardiomyocytes and its effect on hypoxia-induced apoptosis
摘要
Abstract
Objective To investigate the expression of Nogo-A protein in rat cardiomyocytes and its effect on hypoxiainduced apoptosis.Methods ① Nogo-A protein expression was determined in adult rat cardiomyocytes by using immunohistochemistry and Western blot.② The acutely isolated myocardial cells were divided into three groups:the control group,model group and intervention group.To establish the hypoxia-induced myocardial cell apoptosis models in the model group and intervention group,the intervention group was treated with the anti-Nogo-A antibodies before the modeling.The apoptosis,the expre.ssion of Nogo-A protein,Caspase-3 and Caspase-12 in three groups were observed.Resets ① Adult cardiomyocytes:the expression of Nogo-A protein was positive.② Cardiomyocytes under hypoxia:the expression of NogoA protein was positive; the intervention group and the model group appeared apoptotic cells,but the intervention group was significantly less than the model group; the positive rates of Caspase-3 and Caspase-12 expression were significantly higher in the intervention group and model group than those in the control group,but the intervention group was significantly lower than the model group,P ＜ 0.05.Conclusions There was a large amount of expressed Nogo-A protein in myocardial cells.Nogo-A protein may be involved in the hypoxia-induced apoptosis of myocardial cells.关键词
Nogo-A蛋白/心肌细胞/缺氧性凋亡Key words
Nogo-A protein/ cardiomyocytes/ hypoxic apoptosis分类
医药卫生引用本文复制引用
程亮,易蔚,任凯,裴建明..Nogo-A蛋白在大鼠心肌细胞中的表达变化及其在细胞缺氧性凋亡中的作用[J].山东医药,2013,53(22):13-15,21,4.基金项目
陕西省社发公关资助项目(2012K15-01-05). （2012K15-01-05）
