山东医药2013，Vol.53Issue(24)：6-8,后插1,4.DOI:10.3969/j.issn.1002-266X.2013.24.002
乳腺癌组织中LOX的表达变化及其与MMP-2、MMP-9、HIF-1α的关系
Expression of LOX in breast caner tissue and its correlation with MMP-2, MMP-9 and HIF-1α
摘要
Abstract
Alstract:Objective To investigate the expression of lysyl oxidase (LOX) in breast caner tissue and its correlation with MMP-2,MMP-9 and HIF-1α.Methods The expression of LOX mRNA and protein were detected in 120 cases of human breast cancer tissue,the adjacent breast tissues,and 30 cases of breast benign lesion tissue by RT-PCR and immunohistochemical staining respectively.The relationship between the expression of LOX in the breast cancer tissue and lymph node metastasis was analyzed.MMP-2,MMP-9 and HIF-1 α protein were detected by immunohistochemical staining and the correlation of their expression with LOX in breast cancer was conducted.Results The expression level of LOX mRNA in breast cancer tissue was 0.44 ± 0.08,and the positive rate of LOX protein was 67.5％ (81/120) in breast cancer tissue,which were significantly higher than those of the adjacent breast tissues and breast benign lesion tissue(all P ＜0.05).Expression of LOX was related to lymph node metastasis,and the expression of LOX in breast cancer tissue with lymph node metastasis was higher than that without metastasis (P =0.000).The positive rates of MMP-2,MMP-9 and HIF-1 α protein were 70.0％ (84/120),68.3％ (82/120) and 61.7％ (74/120) in breast cancer tissue,which were significantly higher than those in the adjacent breast tissues and breast benign lesion tissue(all P ＜ 0.05).Correlation analysis showed that the expression of LOX was positively correlated with MMP-2,MMP-9 and HIF-1 α (r =0.450,0.423,0.462,respectively;all P ＜ 0.05).Conclsion LOX is highly expressed and related with lymph node metastasis in breast cancer,and it may promote the invasion and metastasis of breast cancer by interacting with MMP-2,MMP-9 and HIF-1α.关键词
乳腺肿瘤/赖氨酰氧化酶/基质金属蛋白酶/低氧诱导因子Key words
breast neoplasms / lysyl oxidase / matrix metalloproteinase/ hypoxia inducible factor分类
医药卫生引用本文复制引用
韦薇,刘剑仑,唐玮,杨华伟,蒋奕,刘起鹏..乳腺癌组织中LOX的表达变化及其与MMP-2、MMP-9、HIF-1α的关系[J].山东医药,2013,53(24):6-8,后插1,4.基金项目
国家自然科学基金资助项目(81160321). （81160321）
