实验动物与比较医学2013，Vol.33Issue(3)：190-194,5.DOI:10.3969/j.issn.1674-5817.2013.03.005
家兔心搏骤停复苏模型的病理生理改变
Physiopathological Changes in Rabbit Model of Cardiac Arrest Resuscitation
摘要
Abstract
Objective To discuss physiopathological changes in a rabbit model of asphyxia induced cardiac arrest and advantages of the model applied to the study of post-cardiopulmonary resuscitation.Methods Fifteen clean-grade adult rabbits were included in this study.Cardiac arrest was induced by asphyxia,the duration of which was 7 minutes.The changes of blood pressure and electrocardiogram were observed before and after cardiac arrest.And the time of cardiac arrest(c)the time to restoration of spontaneous circulation(ROSC)、the time to restoration of spontaneous breath,and the electrocardiogram when cardiac arrest occurred were recorded.The rate of restoration of spontaneous circulation and survival rate in 6 h,12 h,24 h,and 48 h were calculated.The arterial blood gas analysis was tested before and after cardiac arrest.Results ① MAP before closing trachea is 116.18±8.89 mmHg,at the moment of restoration of spontaneous circulation,was 121.03±16.75 mmHg,at 15 minutes since that,was 90.92±13.68 mmHg,and remained at that level thereafter.According to the electrocardiogram recorded at the moment of cardiac arrest,we found 2 cases of ventricular fibrillation,11 cases of electromechanical dissociation,and 2 cases of asystole with a straight line.The electrocardiogram showed the progression of ST segment elevation and recovery from the restoration of spontaneous circulation to 30 minutes.②The rate of restoration of spontaneous circulation from cardiac arrest was 100％(15/15),and the time was 147.60±22.09 s.The time to restoration of spontaneous breath was 403.33±130.18 s.The survival rate in 6h,12h,24h,and 48h were 93.33％(14/15),73.33％(11/15),53.33％(8/15) and 26.67％(4/15) separately.③ At the moment of restoration of spontaneous circulation,pH of the arterial blood gas analysis obviously decreased (P＜0.05),and PaO2、PaCO2 and lactic acid obviously increased (P＜0.05),compared with those before asphyxia.Conclusion The rabbit model of asphyxia induced cardiac arrest can simulate the characteristics of the pathophysiological changes in clinical cardiopulmonary resuscitation.The main adventages include the research needs of laboratory measurements and hemodynamic monitoring,the experimental operation is easy to grasp and without thoracotomy.关键词
心搏骤停/动物模型/窒息法/心肺复苏Key words
Cardiac arrest/ Animal model/ Asphyxia/ Cardiopulmonary resuscitation分类
生物科学引用本文复制引用
张东,李南,李洪祥,王育珊..家兔心搏骤停复苏模型的病理生理改变[J].实验动物与比较医学,2013,33(3):190-194,5.基金项目
国家自然科学基金面上项目(30870351) （30870351）
吉林省科技发展计划项目(200705201) （200705201）
