实验动物与比较医学2013，Vol.33Issue(3)：204-209,6.DOI:10.3969/j.issn.1674-5817.2013.03.008
长爪沙鼠小鼠肝炎病毒抗体ELISA检测方法的建立与初步应用
Establishment and Preliminary Application of ELISA for Detecting Antibody to Mouse Hepatitis Virus in Mongolian Gerbil
摘要
Abstract
Objective To develop a ELISA method for determination of Mouse Hepatitis Virus antibody in Mongolian gerbils.Methods The cultured DBT cell were vaccinated with MHV-V1、MHV-V3、MHV-JHM virus for preparation the normal DBT antigen and special MHV antigen,the best working density of enzyme union and the normal or special antigen were titrated and the experiments of accuracy,sensitivity,stability and specificity were done.Result The best working density of normal,special antigen and the enzyme union is 0.4 μg/ml,5ìg/ml and 1:5000 respectively; The interassay coefficient of variation of normal antigen and special antigen is 7.9％ and 6.8％ respectively,the intra-assay average coefficient of variation is 12.7％ and 9.7％ respectively; The detection sensitivity is 1:1280; There is no cross-reactivity with Sendai virus (SV) and Lymphocytic Choriomeningitis virus (LCMV).The stability test shows the relative deviation is below 25％.Conclusion The ELISA method is good in duplication,stability,specificity,and sensitivity,as a result of which ELISA may be used in detecting the antibody to MHV in Mongolian gerbil.关键词
小鼠肝炎病毒/ELISA(MHV)/长爪沙鼠Key words
Mouse hepatistis virus(MHV)/ ELISA/ Mongolian gerbil分类
生物科学引用本文复制引用
卫礼,王吉,付瑞,李晓波,王淑菁,岳秉飞,贺争鸣..长爪沙鼠小鼠肝炎病毒抗体ELISA检测方法的建立与初步应用[J].实验动物与比较医学,2013,33(3):204-209,6.基金项目
实验动物新品种的种群建立与质量标准化研究(国家科技支撑计划项目:2011BAI15B01) （国家科技支撑计划项目:2011BAI15B01）
