实验动物与比较医学2013，Vol.33Issue(3)：210-214,5.DOI:10.3969/j.issn.1674-5817.2013.03.009
Beagle犬活体取胎及其成纤维细胞的培养
The Foetus of Beagles Obtained in vivo and Fetal Fibroblasts Cultured
摘要
Abstract
Objective To explore some methods of obtaining fetuses of 30 days by surgical operation and the ways of the fetal fibroblasts culture.Methods Identified the estrus of female Beagles for timed mating,then observed development of the fetues to determine the time of pregnancy by B-ultrasonic examination.When the female beagle was 30 days pregnant,removed the fetuses from its one side of the uterus by surgical operation,and then separated fetal fibroblasts with trypsin digestion after removing its head,limbs and internal organs.The cells were cultured and passaged in vitro.Meanwhile,the remaining fetuses of the other side of the uterus were retained,and the female Beagle was nursed carefully to insure development of the fetuses.Results Successfully isolated and cultured the canine fetal fibroblasts,and kept the remaining fetuses developing to full term and survived.Conclusion This experiment reserved the female dog which can be used as receptor for embryo transfer; the remaining pups can be used as the control group of the full-sib relationship for cloning Beagles in the future.关键词
活体/Bealge犬/胎儿成纤维细胞Key words
In vivo/ Beagles/ Fetal fibroblast分类
生物科学引用本文复制引用
张敦伟,王晓民,连雪科,刘运忠,高翔,倪庆纯..Beagle犬活体取胎及其成纤维细胞的培养[J].实验动物与比较医学,2013,33(3):210-214,5.基金项目
十二五国家科技重大专项"实验用Beagle犬及疾病模式犬研究开发平台"[2011ZX09307-304] （）
