生态学杂志2013，Vol.32Issue(4)：1075-1082,8.
一株产生物表面活性剂低温细菌的筛选与鉴定
Isolation and identification of a cold-adapted biosurfactant-producing bacterium
摘要
Abstract
By using blood agar plate and blue agar plate as the primary screening media, and oil spreading as the re-screening method, 4 strains of cold-adapted biosurfactant-producing bacteria were isolated from the straws under natural decay in low temperature environment. Among the four strains, B-17 had the highest oil displacement activity, which could decrease the surface tension of fermentation broth from 75. 47 mN · m-1 to 37. 49 mN · m-1 within 5 days. Through the analyses of morphological, physiological, and biochemical characteristics and 16S rDNA sequence, the strain B-17 was preliminarily identified as Petrimonas sp. The results of FT-IR showed that the biosurfactant produced in the metabolic process of the B-17 was a kind of glyco-lipid. The emulsification activity of the fermentation liquor of the B-17 could maintain at 75% within 5 days, with good solubilizing effect. The optimal culture conditions for the growth of the B-17 and for its producing biosurfactant were initial pH 7, salt concentration 0.4% , and culture temperature 20℃. This study provided a basis for the exploitation of cold-adapted biosurfactant-producing bacteria.关键词
生物表面活性剂/低温细菌分离鉴定/表面张力/乳化Key words
biosurfactant/ isolation and identification of cold-adapted bacteria/ surface tension/ emulsification分类
生物科学引用本文复制引用
刘畅,赵伟,李涛,王宏燕..一株产生物表面活性剂低温细菌的筛选与鉴定[J].生态学杂志,2013,32(4):1075-1082,8.基金项目
国家科技支撑课题项目(2009BADB3B01)、黑龙江省教育厅课题项目(12521020)和黑龙江省教育厅新农村建设研究项目"黑龙江省资源循环型农村建设的对策研究"资助. （2009BADB3B01）
