山西农业大学学报（自然科学版）2013，Vol.33Issue(1)：42-45,4.
蔬菜作物的RNA原位杂交技术
RNA in situ Hybridization in Vegetable Plants
摘要
Abstract
RNA in situ hybridization is one of the most direct and effective ways to explore the temporal and spatial gene expression patterns at the cellular level.RNA in situ hybridization has been widely used in Arabidopsis and maize,but few studies have been reported in vegetable plants,possibly due to its technique difficulties.Combining the RNA in situ hybridization techniques in Arabidopsis and maize with our experimental experience,we optimized the RNA in situ hybridization for vegetable plants.In this study,using cucumber as an example,we described the specific procedures for RNA in situ hybridization and a few remarks for vegetable plants.关键词
RNA原位杂交/基因/时空表达/黄瓜Key words
In situ hybridization/Gene/Temporal and spatial expression/Cucumber分类
农业科技引用本文复制引用
柳美玲,丁莲,张小兰..蔬菜作物的RNA原位杂交技术[J].山西农业大学学报（自然科学版）,2013,33(1):42-45,4.基金项目
中央高校基本科研业务费专项资金项目(2012RC012) （2012RC012）
