现代检验医学杂志2013，Vol.28Issue(3)：32-34,3.DOI:10.3969/j.issn.1671-7414.2013.03.010
河北省发现携带NDM-1型金属β-内酰胺酶基因的阴沟肠杆菌临床分离株
Detection of Carbapenemase NDM-1 in Carbapenem-resistant Enterobacter Cloacae in Hebei ProvinceSHI Dong-yan1,CAO Li-jun2,YANG Jing1,3,LI Zhi-rong1,3,QIANG Cui-xin1,3,ZHAO
时东彦 1曹丽君 2杨靖 1李志荣 3强翠新 1赵建宏3
作者信息
- 1. 河北医科大学第二医院检验科,石家庄,050000
- 2. 河北大学附属医院检验科,河北保定,071000
- 3. 河北省临床检验中心,石家庄,050000
- 折叠
摘要
Abstract
Objective To investigate the resistance mechanism of carbapenem resistant Enterobacter cloacae Enterobacteriacea in hebei antimicrobiolmicrobiol resistant net. Methods The Enterobacteriaceae isolates resistant to carbapenemase were collected from October of 2011 to October of 2012. Antimicrobiol resistant test were determinated by K-B test and E-test. Isolates were screened for phenotype by modified Hodge test and metalloenzyme test. PCR amplification was used to analiyze the existence of β-lactamase gene. Reults A total of 50 strains of Enterobacteriaceae resistant to carbapenemase were isolated ,1 of 50 was Enterobacter cloacae. The inhibitory diameter of Imipenem and Meropenem againistst Entobacter cloacae was 6mm by K-B method, MIC of Imipenem and Meropenem was > 32 jug/ml by E-test. Modified Hodge test showed posi-tivenegative and,but metalloenzyme test was positive. β-lactamase gene NDM-1 were identified by PCR and sequence analysis was 99% similarity to NDM-1 by GeneBank. Conclusion β-lactamase gene NDM-1 were firstly identified in Enterobacter cloacae in Hebei province.关键词
阴沟肠杆菌/碳青霉烯类抗生素/NDM-1Key words
Enterobacter cloacae/ carbapenems/ NDM-1分类
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时东彦,曹丽君,杨靖,李志荣,强翠新,赵建宏..河北省发现携带NDM-1型金属β-内酰胺酶基因的阴沟肠杆菌临床分离株[J].现代检验医学杂志,2013,28(3):32-34,3.
