实用肝脏病杂志2013，Vol.16Issue(2)：137-140,4.DOI:10.3969/j.issn.1672-5069.2013.02.015
黄连素灌胃对非酒精性脂肪性肝炎小鼠肠道菌群的影响
Modulation of gut microbiota with berberine improves nonalcoholic steatohepatitis in mice
摘要
Abstract
Objective To assess whether the gut microbiota is involved in the pathophysiology of nonalcoholic steatohepatitis (NASH).Methods Babl/c mice were randomly divided into control(n=10),model(n=10)and the therapeutic group (n=1S).The mice in control group were fed with normal diet and in other two groups were fed with high-fat diet.Meanwhile,the therapeutic group were treated with berberine (200mg.kg-1)since fifth week.The mice were sacrificed at the end of thirteen week.We assessed changes in bifidobacteria and lactobacillus in fecal contents and hepatic CD14,IL-1,IL-6 and TNF-α mRNA were detected by PCR.Results At the end of thirteenth week,the degree of steatosis and inflammation were more serious in the model group with the NAFLD activity score of 4.60±l.07,much higher than in the control (0.44±0.53,P＜0.01);serum ALT in model group was obviously increased as compared with that in control group (346.00±142.01U/L vs.120.00±l0.22U/L,P＜0.01);the hepatic levels of IL-1,IL-6 and TNF-α mRNA in therapeutic group were downregulated as compared with in model group[0.243±0.067 vs.1.539±0.437(P＜0.01),0.249±0.046 vs.0.580-±0.083(P＜0.01),and 0.553±0.553 vs.4.482±0.820 (P＜0.01)];the bifidobacteria and lactobacillus counts in fecal contents in berberine-treated group significantly improved as compared with in model group,especially for bifidobacteria (2.160±0.629 vs.0.495±0.082,P＜0.01).Conclusions The finding in our study demonstrates that berberine could modulate the microbiota in gut,which is conceivable that the strategies for changing gut microbiota would improve NASH.关键词
非酒精性脂肪性肝炎/肠道菌群/黄连素/Babl/c小鼠Key words
Nonalcoholic steatohepatitis/ Gut/ Microbiota/ Berberine/ Mice引用本文复制引用
曹毅,徐雷鸣,潘勤,王晓颖,沈峰,陈光榆,范建高..黄连素灌胃对非酒精性脂肪性肝炎小鼠肠道菌群的影响[J].实用肝脏病杂志,2013,16(2):137-140,4.基金项目
国家自然科学基金面上项目(编号:81070322) （编号:81070322）
国家科技部973项目课题(编号:2012CB517501) （编号:2012CB517501）
上海市科委重点科技攻关项目(编号:10411956300,09140903500) （编号:10411956300,09140903500）
