土壤2013，Vol.45Issue(2)：271-276,6.
产ACC脱氨酶植物根际促生菌的筛选及其对修复植物高羊茅生长的影响
Isolation of Plant Growth Promoting Rhizobacteria Produced ACC Ammonialyase and Its Influence on Tall Fescue Growth
摘要
Abstract
In this study,the strains with 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase activity were isolated from an oil-polluted soil.The ACC deaminase activity of the strain D5A was 0.084 U/mg in minimal medium containing ACC as the sole nitrogen source.In addition,D5A also had properties of producing indole acetic acid (IAA),salt tolerance and dissolving phosphorus.In liquid culture conditions,the strains produced IAA up to 112 mg/L.It was still able to grow normally in the medium of 90 g/kg salt and had a strong ability to dissolve the mineral phosphorus.The strains grew well in LB medium from the initial pH value of 4 to 10,which showed the strains had good adaptability of pH.Seed germination tests showed that the strains could significantly improve seed germination and bud of tall fescue(Festuca arundinacea) at adverse conditions of 3 g/kg and 6 g/kg NaCl.Finally,based on physiological,biochemical characteristics and 16S rDNA sequence analysis,the strains was identified as Klebsiella sp.关键词
高羊茅/ACC脱氨酶/IAA/铁载体/溶磷Key words
Tall fescue/ ACC deaminase/ IAA/ Siderophore/ Dissolved phosphate分类
生物科学引用本文复制引用
丁琳琳,刘五星,孙剑英,骆永明,徐旭士,李振高..产ACC脱氨酶植物根际促生菌的筛选及其对修复植物高羊茅生长的影响[J].土壤,2013,45(2):271-276,6.基金项目
国家自然科学基金项目(41001182)和环境保护公益性行业科研专项基金项目(201009015)资助. （41001182）
