西安交通大学学报（医学版）2013，Vol.34Issue(4)：479-482,4.
5-氮杂-2'-脱氧胞苷对HepG2中抑癌基因MIA2表达的影响
Effect of 5-Aza-2'-deoxycytidine on the expression of tumor suppressor gene MIA2 in HepG2
摘要
Abstract
Objective To observe the effect of methylase inhibitor 5-Aza-2'-deoxycytidine (5-Aza-CdR) on the proliferation and apoptosis of HCC cell line HepG2 and detect the expression of MIA2 protein in HepG2 after 5-Aza-CdR treatment. Methods The proliferation and apoptosis of HepG2 treated with 5-Aza-CdR of 0, 1, 2, 4, 8, 10, 20 and 40 μmol/L were detected by MTT and flow cytometry assay. Then after HepG2 was treated with 0 μmol/L and 10 μmol/L of 5-Aza-CdR, the MIA2 protein expression was detected by immunohistochemistry and Western blot. Results MTT showed that after treatment with a certain concentration range of 5-Aza-CdR, the proliferation of HepG2 was inhibited and there was a positive correlation between cell proliferative inhibition rate and drug concentration. Flow cytometry showed that the apoptosis of HepG2 was increased and a positive correlation existed between early apoptosis rate and drug concentration. Immunohistochemistry and Western blot revealed that the level of MIA2 protein was elevated in 10 μmol/L group compared with that in control group (0 μmol/L). Conclusion After HepG2 is treated with 5-Aza-CdR, MIA2 protein expression is enhanced, cell proliferation is inhibited and early apoptosis is promoted.关键词
5-氮杂-2'-脱氧胞苷/黑色素瘤抑制蛋白2(MIA2)/肝癌细胞/凋亡Key words
5-Aza-2'-deoxycytidine/ melanoma inhibitory activity 2 (MIA2)/ liver cancer cell/ apoptosis分类
医药卫生引用本文复制引用
杨少奇,姚文暾,杨花,黄睿,胡建国,何芳..5-氮杂-2'-脱氧胞苷对HepG2中抑癌基因MIA2表达的影响[J].西安交通大学学报（医学版）,2013,34(4):479-482,4.基金项目
国家自然科学基金资助项目(No.81060194) （No.81060194）
