西安交通大学学报（医学版）2013，Vol.34Issue(4)：483-486,4.
脑缺血预处理对大鼠脑内Nogo-A mRNA、Nogo-A及NgR蛋白表达的影响
Effects of ischemic preconditioning on the expressions of Nogo-A mRNA, Nogo-A and NgR protein after focal cerebral ischemia-reperfusion injury in adult rats
焦义明 1王金兰 1娄季宇 1白宏英1
作者信息
- 1. 郑州大学第二附属医院神经内科,河南郑州,450014
- 折叠
摘要
Abstract
Objective To investigate the effects of ischemic preconditioning (IP) on the expressions of Nogo-A mRNA, Nogo-A and NgR protein after focal cerebral ischemia-reperfusion injury in adult rats. Methods A total of 152 adult male SD rats were randomly divided into three groups: control group (n = 8), non-ischemic preconditioning group (NIP, n = 72) and IP group (n = 72). The latter two groups were randomly divided into day-1, day-3 and day-7 subgroups (24 in each subgroup). Middle cerebral artery occlusion model was induced by intraluminal suture method. After 10 minutes of IP, 2-hour re-ischemia and 1-day reperfusion were performed at day 1, day 3 and day 7. The brain infarct volume was evaluated with 2,3,5-triphenyltetrazolium chloride (TTC). The expressions of Nogo-A mRNA in the three groups were detected by RT-PCR method whereas the positive expressions of Nogo-A and NgR protein in the ischemic cerebral cortex were observed by immunohistochemical method. Results Compared with those in the corresponding NIP subgroups, the neurological deficit score and brain infarct volume were decreased significantly, and the expressions of Nogo-A mRNA, Nogo-A and NgR protein were downregulated significantly in IP day-1, day-3 and day-7 subgroups (all P<0.05). The brain infarct volume as well as Nogo-A mRNA, Nogo-A and NgR protein expressions were decreased most significantly in IP day-3 subgroup (P<0.05), but they did not differ significantly in day-1 and day-7 subgroups (P>0. 05). Conclusion The downregulated Nogo-A and NgR expressions induced by cerebral ischemic preconditioning may play an important role in brain ischemic tolerance.关键词
脑缺血预处理/Nogo-A mRNA/Nogo-A/NgR/脑缺血耐受/脑梗死Key words
cerebral ischemic preconditioning/ Nogo-A mRNA/ Nogo-A/ NgR/ brain ischemic tolerance/ brain infarct分类
医药卫生引用本文复制引用
焦义明,王金兰,娄季宇,白宏英..脑缺血预处理对大鼠脑内Nogo-A mRNA、Nogo-A及NgR蛋白表达的影响[J].西安交通大学学报（医学版）,2013,34(4):483-486,4.
