西南国防医药2013，Vol.23Issue(6)：586-588,3.DOI:10.3969/j.issn.1004-0188.2013.06.003
尿液分离大肠埃希菌株耐药性及生物被膜形成分析
Drug resistance of escherichia coli strains isolated from urine specimens and their ability of biofilm formation
邹自英 1熊杰 1朱冰 1连丽莎 1汪璐 1曾平1
作者信息
- 1. 610083,成都,成都军区总医院检验科
- 折叠
摘要
Abstract
Objective To discuss the detection rate of escherichia coli ( ECO) strains isolated from urine extended β-actamases ( ESBLs) in patients with urinary tract infection , their drug resistance, and their ability of biofilm formation. Methods VITEK2-COMPACT microbe analysator was used to identify the ECO strains isolated from urine specimens which were clinically collected from October 2010 to December 2011 and determined their drug susceptibility. Crystal violet semiquantitative method was used to detect the ability of biofilm formation. Results Among the 115 strains of ECO isolated from urine specimens , there were 68 strains (59. 13% ) producing ESBLs and 47 strains(40. 87% )which did not produce ESBLs. Eighty two strains of ECO(71. 30% )were resistant to more than three kinds of antibiotics. Among these strains ,64 ones produced ESBLs which accouted for 55. 65% of the total number of stains and 94.12% of the strains producing ESBLs. The resistant rate to the antibiotics below was the highest : ampicillin (88. 70% ) and cefazolin(65. 22% ). Both resistant rates to ciprofloxacin and levofloxacin were more than 60%. For commonly used penicillins, Ⅲ generation of cephalosporins , fluoroquinolones , tobramycin, gentamicin, monobactams, and sulfanilamide antibiotics ,the MIC value and the drug resistant rate of the strains producing ESBLs significantly increased compared with those of the strains not producing ESBLs (P<0.05 or P<0.01). There was no significant difference in the susceptibility between the strains producing and not producing ESBLs,as to the antibiotics such as cefotetan ,cefepime,nitrofurans,amikacin,piperacillin/tazobactam,and carbapenems. 98. 26% of the isolated strains had the ability of biofilm formation . Conclusion The proportion of strains producing ESBLs is high in the isolatied ECO from urine specimens ,and they have strong ability of biofilm formation. They have the characteristic of multi-drug resistance.关键词
尿/大肠埃希菌/超广谱β-内酰胺酶/生物被膜/耐药性Key words
urine /escherichia coli/extended β-actamases/biofilm/drug resistance分类
医药卫生引用本文复制引用
邹自英,熊杰,朱冰,连丽莎,汪璐,曾平..尿液分离大肠埃希菌株耐药性及生物被膜形成分析[J].西南国防医药,2013,23(6):586-588,3.
