烟草科技Issue(6)：53-56,4.
烟草及其制品中砷的形态分析
Speciation Analysis of Arsenic in Tobacco and Tobacco Products
摘要
Abstract
In order to determine the chemical species of arsenic (As) in tobacco samples,a method combining ultrasonic solvent extraction and high performance liquid chromatography (HPLC) with inductively coupled plasma-mass spectrometry (ICP-MS) was developed to separate and analyze six species of arsenic,including arsenous acid [As(Ⅲ)],arsenic acid [As(V)],dimethylarsinic acid (DMA),monomethylarsonic acid (MMA),arsenobetaine (AsB),and arsenocholine (AsC).The result showed that the baselines of the six species of arsenic mentioned above were separated within 15 mins by Hamilton PRPX-100 (250 × 4.1 mm,10 μm) anion exchange column and using 15 mmol/L (NH4)2HPO4 (pH6.0) as the mobile phase.The linear ranges were no less than two orders of magnitude.The detection limit ranged from 0.44 to 0.98 μg/L and the recoveries were from 85.19％ to 87.31％ with the RSD (n=5) of 2.45％ to 3.56％.The content of As in tobacco was low,and some arsenic species existed in the forms of DMA and MMA were of lower toxicity.Ultrasonic solvent extraction could extract the organic and inorganic As in tobacco simultaneously and reflect the distribution of As species in tobacco samples objectively.This method is accurate,reliable,and suitable for the speciation analysis of As in tobacco,and can provide a reference for the safety evaluation of tobacco.关键词
烟草/砷(As)/形态分析/超声溶剂提取/高效液相色谱/电感耦合等离子体质谱(ICP-MS)Key words
Tobacco/ Arsenic/ Speciation analysis/ Ultrasonic solvent extraction/ HPLC/ ICP-MS分类
轻工纺织引用本文复制引用
张建平,刘秀彩,黄朝章,刘泽春,许寒春,谢卫..烟草及其制品中砷的形态分析[J].烟草科技,2013,(6):53-56,4.基金项目
国家烟草专卖局卷烟减害技术重大专项子项目"'七匹狼'卷烟主流烟气7种成分释放量影响因素研究"(110200902013). （110200902013）
