烟台大学学报（自然科学与工程版）2013，Vol.26Issue(3)：178-181,4.
玻璃纤维素壳聚糖膜固定乙酰胆碱酯酶的研究
Immobilization of Acetylcholinesterase with Chitosan Coated Glass Fibre Membrane
摘要
Abstract
Acetylcholinesterase is immobilized on chitosan coated glass fibre membrane using glutaraldehyde as cross-linking reagent.The benificial immobilizing conditions are obtained as follows:1 mL of enzyme solution is prepared with 0.2 mol/L phosphate buffer(pH 8.0) adding 50 μL 150 U/mL AChE solution,50 μL 5％ (V/V)glutaraldehyde and 100 μL 1％ (W/W) BSA.The chitosan coated glass fibre membrane is immersed in the enzyme solution for 8 h at 4 ℃.The optimum temperature and pH value of immobilized AChE are 37 ℃ and 8.0,and the immobilized AChE could be reused four times.关键词
玻璃纤维素壳聚糖膜/乙酰胆碱酯酶/固定化Key words
chitosan coated glass fibre membrane/ acetylcholinesterase/ immobilization分类
矿业与冶金引用本文复制引用
曲慧,车育,姜爱莉,于贞..玻璃纤维素壳聚糖膜固定乙酰胆碱酯酶的研究[J].烟台大学学报（自然科学与工程版）,2013,26(3):178-181,4.基金项目
山东省自然科学基金资助项目(ZR2011DL008,Y2008D48). （ZR2011DL008,Y2008D48）
