应用生态学报2013，Vol.24Issue(6)：1639-1646,8.
呼伦贝尔沙地不同植被恢复模式对土壤固氮微生物多样性的影响
Effects of different vegetation restoration patterns on the diversity of soil nitrogen-fixing microbes in Hulunbeier sandy land, Inner Mongolia of North China
摘要
Abstract
By using polymerase chain reaction-denaturing gradient gel electrophoresis (PCRDGGE) and sequence analysis,this paper studied the nifH gene diversity and community structure of soil nitrogen-fixing microbes in Hulunbeier sandy land of Inner Mongolia under four years management of five vegetation restoration modes,i.e.,mixed-planting of Agropyron cristatum,Hedysarum fruticosum,Caragana korshinskii,and Elymus nutans (ACHE) and of Agropyron cristatum and Hedysarum fruticosum (AC),and mono-planting of Caragana korshinskii (UC),Agropyron cristatum (UA),and Hedysarumfruticosum (UH),taking the bare land as the control (CK).There existed significant differences in the community composition of nitrogen-fixing microbes among the five vegetation restoration patterns.The Shannon index of the nifHgene was the highest under ACHE,followed by under AC,UC,UA,and UH,and the lowest in CK.Except that UH and CK had less difference in the Shannon index,the other four vegetation restoration modes had a significantly higher Shannon index than CK (P＜0.05).The phylogenetic analysis showed that the soil nitrogen-fixing microbes under UA,UH,and UC were mainly of cyanobacteria,but the soil nitrogen-fixing microbes under AC and ACHE changed obviously,mainly of proteobacteria,and also of cyanobacteria.The canonical correlation analysis showed that the soil total phosphorus,available phosphorus,total nitrogen,and nitrate nitrogen contents under the five vegetation restoration modes had significant effects on the nitrogen-fixing microbial communities,and there existed significant correlations among the soil total phosphorus,available phosphorus,total nitrogen,and nitrate nitrogen.It was suggested that the variations of the community composition of soil nitrogen-fixing microbes under the five vegetation restoration modes were resulted from the interactive and combined effects of the soil physical and chemical factors.关键词
呼伦贝尔沙地/植被恢复/nifH基因/变性梯度凝胶电泳/群落多样性Key words
Hulunbeier sandy land/ vegetation restoration/ nifH gene/ denaturing gradient gel electrophoresis (DGGE) / community diversity.分类
生物科学引用本文复制引用
李刚,王丽娟,李玉洁,乔江,张海芳,宋晓龙,杨殿林..呼伦贝尔沙地不同植被恢复模式对土壤固氮微生物多样性的影响[J].应用生态学报,2013,24(6):1639-1646,8.基金项目
国家自然科学基金项目(31170435,31000242)资助. （31170435,31000242）
