中国病理生理杂志2013，Vol.29Issue(6)：1014-1019,6.DOI:10.3969/j.issn.1000-4718.2013.06.010
沉默Notch1基因促进人乳腺癌MCF-7细胞JNK1和p53磷酸化
Notch-1 gene silencing promotes phosphorylations of JNK1 and p53 in human breast cancer MCF-7 cells
摘要
Abstract
AIM:To investigate the effect of Notch1 gene silencing on phosphorylations of JNK1 and p53 in human breast cancer MCF-7 cells.METHODS:shRNA-Notch1 eukaryotic expression plasmid was constructed and transfected into MCF-7 cells.The expression of Notch1 and Hes-1 was observed by Western blotting after transfction.Apoptosis and mitochondrial membrane potential were detected by flow cytometry.Western blotting was also used to determine the protein levels of p-JNK1,p-p53,PUMA,NOXA and cleaved caspase-3 after Notch1 silencing was performed in MCF-7 cells.RESULTS:Silencing of Notch1 significantly reduced the expression of Notch1 and Hes-1 in MCF-7 cells (P ＜0.01).In shNotch1 group,the number of apoptotic cells was much higher (P ＜ 0.01) and mitochondrial membrane potential was much lower (P ＜ 0.05) than those in shControl group.The protein levels of p-JNK1,p-p53,PUMA,NOXA and cleaved caspase-3 increased obviously after silencing of Notch1 was performed in MCF-7 cells (P ＜ 0.05).CONCLUSION:Notch1 silencing induces apoptosis of human breast cancer MCF-7 cells through promoting phosphorylations of JNK1 and p53,and increasing the production of PUMA,NOXA and cleaved caspase-3.关键词
Notch1蛋白/短发夹RNA/JNKI蛋白/p53蛋白/MCF-7细胞Key words
Notch1 protein/Short hairpin RNA/JNK1 protein/p53 protein/MCF-7 cells分类
医药卫生引用本文复制引用
袁磊,陈旭东,范文娟,杨旭光,王建国..沉默Notch1基因促进人乳腺癌MCF-7细胞JNK1和p53磷酸化[J].中国病理生理杂志,2013,29(6):1014-1019,6.基金项目
河南省基础与前沿技术研究计划项目(No.122300410277) （No.122300410277）
漯河医学高等专科学校科研基金资助项目(No.2010-S10) （No.2010-S10）
