中国病理生理杂志2013，Vol.29Issue(6)：1029-1033,5.DOI:10.3969/j.issn.1000-4718.2013.06.013
EGFR-p38 MAPK信号通路参与机械通气肺损伤大鼠肺组织HMGB1的表达
EGFR-p38 MAPK signaling pathway is involved in expression of HMGB1 in pulmonary tissues of rats with ventilator-induced lung injury
摘要
Abstract
AIM:To investigate the role of epidermal growth factor receptor (EGFR)-p38 mitogen-activated protein kinase (MAPK) pathway in the expression of high mobility group box 1 protein (HMGB1) in the lung tissues of rats with ventilator-induced lung injury (VILI).METHODS:Thirty-two healthy Sprague-Dawley (SD) rats were randomly divided into 4 groups (n =8 each):group A,spontaneous breathing; group B,small tidal volume ventilation (VT =8 mL/ kg) ; group C,high tidal volume ventilation (VT =40 mL/kg) ; group D,high tidal volume ventilation plus EGFR antagonist AG-1478.The rats in group B,group C and group D were mechanically ventilated for 4 h and then all animals were sacrificed.Total protein content and white blood cell (WBC) count in bronchoalveolar lavage fluid (BALF),the lung wet/ dry weight ratio (W/D) and myeloperoxidase (MPO) activity were determined.The histological changes of lung tissues were observed by HE staining.The EGFR protein and mRNA expression,p38 MAPK activity and HMGB1 protein expression in the lung tissues were also detected.RESULTS:The inflammatory responses as evidenced by lung HE staining,total protein and WBC in BALF,the lung W/D and MPO activity were significantly higher in group C than those in group A (P ＜ 0.05).The mRNA expression of EGFR,EGFR activity,p38 activity and HMGB1 protein level also significantly increased in group C (P ＜ 0.05) as compared with group A.Significant decreases in the above indexes in group D were observed as compared with group C.CONCLUSION:High tidal volume ventilation induces acute lung injury,which may be related to up-regulation of HMGB1 expression through EGFR-p38 MAPK signal pathway.关键词
机械通气/急性肺损伤/受体,表皮生长因子/高迁移率族盒蛋白1/丝裂原激活蛋白激酶类Key words
Mechanical ventilation/Acute lung injury/Receptors, epidermal growth factor/High mobility分类
医药卫生引用本文复制引用
唐春林,丁宁,程傲冰..EGFR-p38 MAPK信号通路参与机械通气肺损伤大鼠肺组织HMGB1的表达[J].中国病理生理杂志,2013,29(6):1029-1033,5.基金项目
国家自然科学基金资助项目(No.81272136) （No.81272136）
广东省科技计划项目(No.2010B031600011) （No.2010B031600011）
广东省医学科研基金资助项目(No.A2012278) （No.A2012278）
广州市科技计划重点项目(No.2012J4100034) （No.2012J4100034）
广州市医药卫生科技重点项目(No.20121A021001) （No.20121A021001）
