中国病理生理杂志2013，Vol.29Issue(6)：1053-1058,6.DOI:10.3969/j.issn.1000-4718.2013.06.017
α7nAChR的激活通过抑制TNF-α表达促进糖尿病小鼠伤口愈合
Activation of a7nAChR promotes wound healing in diabetic mice by suppressing TNF-a expression
摘要
Abstract
AIM:To explore the role of α7 nicotinic acetylcholine receptor (α7nAChR)-specific agonist PNU-282987 in promoting wound healing in diabetic mice by suppressing the expression of tumor necrosis factor α (TNF-α).METHODS:A model of incised wound was established in diabetic mice or normoglycaemic mice (control).Skin samples were taken on 1 d,3 d,5 d,10 d,14 d and 21 d post-injury (5 mice in each posttraumatic interval).The numbers of macrophages and fibroblasts,the expression of TNF-α and the deposition of collagen were detected by the methods of immunohistochemistry,Western blotting and Masson staining,respectively.After incised wound was performed in the diabetic mice,PNU-282987 was applied by intraperitoneal injection at suitable posttraumatic interval.The above indexes were investigated again.RESULTS:Compared with control group,the diabetic mice presented delayed wound healing.In diabetic mice,the infiltration of macrophages and the expression of TNF-α were significantly reduced in the early phase during wound healing,while they were significantly increased from 5 d post-injury.Besides,from 5 d to 21 d post-injury,the wounds in diabetic mice showed decreased number of fibroblasts and deposition of collagen.From 5 d post-injury,PNU282987 was applied to diabetic mice,which significantly down-regulated the expression of TNF-α,and increased the number of fibroblasts and the content of collagen in the wounds,eventually promoted wound healing.CONCLUSION:Inflammatory reactions delay wound healing in diabetic mice.Activation of α7nAChR promotes wound healing in diabetic mice by suppressing the expression of TNF-α.关键词
糖尿病/创面愈合/α7烟碱型乙酰胆碱受体/肿瘤坏死因子αKey words
Diabetes mellitus/Wound healing/α7 nicotinic acetylcholine receptor/Tumor necrosis factor α分类
医药卫生引用本文复制引用
范琰琰,叶光华,林刻智,董缪武,冯相平,韩军鸽,李兴彪,喻林升..α7nAChR的激活通过抑制TNF-α表达促进糖尿病小鼠伤口愈合[J].中国病理生理杂志,2013,29(6):1053-1058,6.基金项目
浙江省自然科学基金资助项目(No.Q13H150005) （No.Q13H150005）
温州医学院科研发展基金资助项目(No.QTJ12002) （No.QTJ12002）
