中国病理生理杂志2013，Vol.29Issue(6)：1102-1113,12.DOI:10.3969/j.issn.1000-4718.2013.06.025
侧壁开窗法上颌窦底提升术植入Bio-Oss后骨生成的mRNA表达谱
Transcriptional profiling of bone formation after Bio-Oss implantation in maxillary sinus floor elevation by lateral window approach
摘要
Abstract
AIM:To explore the characteristics of transcriptome associated with the unique guided bone regeneration (GBR) of maxillary sinus floor elevation by the lateral window approach.METHODS:One side of the upper jaw was randomly selected for maxillary sinus floor elevation with Bio-Oss by the lateral window approach in 3 Beagle dogs.The other side of maxillary sinus was elevated by the same approach 2 weeks later.Both Bio-Oss particles and ingrown tissueswere harvested 2 weeks after the second operation.Total RNA was extracted and microarray analysis was carried out to identify the differences of the transcriptome at the 2nd and 4th weeks.Functional classification by gene ontology (GO) and signaling pathway analysis by Kyoto Encyclopedia of Genes and Genomes (KEGG) were applied to determine the differentially expressed genes.RESULTS:The genes up-regulated at the 2nd week were associated with stimulus response,immuno-inflammatory response and cell proliferation.Moreover,the genes related to neuron differentiation,osteoclast differentiation and early vascularization process were also up-regulated at this time point.At the 4th week,up-regulated genes were associated with cellular metabolism and development,importantly including angiogenesis and skeletal development.The genes associated with skeletal development were growth and differentiation factors [transforming growth factor beta 1 (TGFB1) and bone morphogenetic protein 4 (BMP4)],extracellular matrix proteins [chondroadherin (CHAD),collagen type Ⅰ,alpha 1 chain (COL1A1),collagen type Ⅰ,alpha 2 chain (COL1A2),collagen type Ⅴ,alpha 2 chain (COL5A2),decorin (DCN),osteonectin (SPARC) and osteocalcin (BGLAP)] and so on.Furthermore,it was found that some of the up-regulated genes at the 4th week were associated with the signaling of transforming growth factor beta/bone morphogenetic protein (TGF-β/BMP) shown by KEGG analysis.The upstream genes of wingless-type MMTV integration site family (WNT) signaling,WNTSA and frizzled receptor 9 (FZD9),were up-regulated at the 2nd week,while the important downstream gene catenin beta 1 (CTNNB1) was up-regulated at the 4th week.CONCLUSION:After 2 weeks of maxillary sinus floor elevation by the lateral window approach,the transcriptome is characterized by down-regulation of the genes associated with immuno-inflammatory response and bone resorption,and up-regulation of the genes associated with osteogenesis.Neurogenesis and angiogenesis are closely related to bone regeneration.The TGF-β/BMP and WNT signaling pathways play an important role in the bone regeneration process.The effect of WNT signaling is complicated.关键词
上颌窦底提升/引导骨组织再生/成骨/转录组/TGF-β/BMP信号通路Key words
Maxillary sinus floor elevation/Guided bone regeneration/Osteogenesis/Transcriptome/TGF-β/BMP signaling pathway分类
医药卫生引用本文复制引用
荣琼,朱双喜,陈松龄,李祥,黄代营,张兴..侧壁开窗法上颌窦底提升术植入Bio-Oss后骨生成的mRNA表达谱[J].中国病理生理杂志,2013,29(6):1102-1113,12.基金项目
广东省科技厅基金资助项目(No.2009B050700025 （）
No.2010B060900070 （）
No.2011B010200008) （）
广东省自然科学基金博士科研启动基金资助项目(No.S201140002559) （No.S201140002559）
