中国动脉硬化杂志2013，Vol.21Issue(6)：481-485,5.
培哚普利改善糖尿病大鼠急性心肌梗死后内皮祖细胞动员
Perindopril Improves Endothelial Progenitor Cell Mobilization in Diabetic Rats After Acute Myocardial Infarction
摘要
Abstract
Aim To investigate the beneficial effects of perindopril on bone marrow-endothelial progenitor cell (EPC) mobilization,neovascularization and cardiac function in diabetic rats after acute myocardial infarction (AMI),and explore the potential underling mechanism for these effects.Methods High fat diet combined with a low dose of Streptozocin (STZ) was used to induce diabetic models,then left anterior descending coronary artery ligation was performed to induce AMI.Diabetic rats were randomly assigned into perindopril group or control group after surgery (n =15 in each group).The percentage of CD45-/1ow + CD133 + KDR + EPC in peripheral blood mononuclear cells was measured by flow cytometry pre-operation and at day1,3,5,7,14,28 post-operation,and the plasma level of vascular endothelial growth factor (VEGF) at the same time points was measured by enzyme linked immunosorbent assay (ELISA) kit.Capillary density in the peripheral area of infarction was determined by CD31 staining.Echocardiagraphy was performed to evaluate cardiac function.The expression and phosphorylation of protein associated with EPC mobilization in bone marrow were determined by Western blot analysis.Results Perindopril treatment could notably improve the impaired ischemia-induced EPC mobilization in diabetic condition,and elevate the tiptop of circulating EPC (103 + 37/106 vs 58 ± 19/106,P ＜0.05).At the same time,the level of plasma VEGF was raised,the expression of Akt and endothelial nitric oxide synthase (eNOS)pbosphorylation,as well as matrix metalloproteinase-9 (MMP-9) expression in bone marrow were increased (all P ＜ 0.05).Compared with control ones,diabetic rats with perindopril treatment had higher capillary density around area of infarction.Moreover,left ventricular ejection fraction and left ventricular fractional shorting were apparently improved in perindopril group (all P ＜ 0.05).Conclusion Perindopril treatment improves the ischemia induced EPC mobilization and neovascularization in diabetic rats,and ameliorates the cardiac function after AMI in diabetic condition,which might be associated with the activation of protein in EPC mobilization pathway.关键词
培哚普利/内皮祖细胞/血管新生/糖尿病/急性心肌梗死/心功能Key words
Perindopril/ Endothelial Progenitor Cell/ Neovascularization/ Diabetes / Acute Myocardial Infarction/ Cardiac Function分类
医药卫生引用本文复制引用
孙佳音,康丽娜,徐标..培哚普利改善糖尿病大鼠急性心肌梗死后内皮祖细胞动员[J].中国动脉硬化杂志,2013,21(6):481-485,5.基金项目
国家自然科学基金(81070195,81000055)和江苏省自然科学基金(BK 2010107) （81070195,81000055）
