中国动脉硬化杂志2013，Vol.21Issue(6)：518-522,5.
氧化型低密度脂蛋白下调Rab4表达诱导人脐静脉内皮细胞自噬
Oxidized Low Density Lipoprotein Induces Human Vascular Umbilical Vein Endothelial Cells Autophagy via Down-regulation of Rab4 Expression
占凡 1刘厂辉1
作者信息
- 1. 南华大学附属第一医院心血管内科,湖南省衡阳市421001
- 折叠
摘要
Abstract
Aim To explore the effect of oxidized low density lipoprotein (ox-LDL) on human vascular umbilical vein endothelial cell (HUVEC) autophagy and its mechanism.Methods After treatment with ox-LDL for 24 h,Western blot was used to detect the protein expression of Beclin-1 and microtubule-associated protein 1 light chain 3 (LC3) ; Reverse transcription polymerase chain reaction (RT-PCR) and Western blot were used to detect Rab4 mRNA and protein expression.After transfection with Rab4 siRNA and Rab4 plasmid,Western blot was used to measure the protein levels of Rab4,Beclin-1 and LC3 induced by 75 mg/L ox-LDL.Results After treatment with ox-LDL for 24 h,the protein expression of Beclin-1 and the ratio of LC3 Ⅱ/LC3 I were increased.The levels of Rab4 mRNA and protein were obviously decreased with a concentrafion-dependent manner.When transfection with Rab4 siRNA,the protein level of Beclin-1 and the ratio of LC3 Ⅱ/LC3 Ⅰ induced by ox-LDL were further increased.When transfection with Rab4 plasmid,the increased protein level of Beclin-1 and the ratio of LC3 Ⅱ/LC3 Ⅰ induced by ox-LDL were attenuated.Condusion ox-LDL increased HUVEC autophagy via down-regulation of Rab4 expression.关键词
氧化型低密度脂蛋白/Rab4/血管内皮细胞/自噬Key words
Oxidized Low Density Lipoprotein/ Rab4/ Vascular Endothelial Cell/ Autophagy分类
医药卫生引用本文复制引用
占凡,刘厂辉..氧化型低密度脂蛋白下调Rab4表达诱导人脐静脉内皮细胞自噬[J].中国动脉硬化杂志,2013,21(6):518-522,5.
