中国动脉硬化杂志2013，Vol.21Issue(6)：549-553,5.
Moesin抗体与动脉粥样硬化相关疾病的关系
Discussion on the Relationship Between Momesin Antibody and Atherosclerosis-related Diseases
毛华 1杜峰 1胥勇 1朱晋坤 1文美 1刘廷筑1
作者信息
- 1. 贵阳市第一人民医院心内科,贵州省贵阳市550002
- 折叠
摘要
Abstract
Aim Obtain purified moesin protein by recombinant,purified recombinant moesin protein was plated as coating antigen of indirect ELISA.And discussion on the relationship between momesin antibody and atherosclerosisrelated diseases.Methods A pair of primers were designed according to the sequence of the moesin.The moesin gene was amplified by PCR.PCR products were then inserted into a prokaryotic expression plasmid,Using isopropyl thiogalactoside (IPTG) induced expression in the appropriate temperature and conditions.Recombinant moesin protein was analyzed by SDS-PAGE.Recombinant moesin protein will be purified.Purified recombinant moesin protein was applied to establish the indirect ELISA.Adsorption assay in 120 patients (20 patients with acute coronary syndrome,30 patients with primary hypertension,20 patients with carotid atherosclerosis,20 patients with diabetes and 30 patients with hyperlipidemia) with diseases related to atherosclerosis anti-moesin antibody by ELISA,compared the positive rate of anti-moesin antibody of blood.Results The expression product was recombinant moesin by SDS-PAGE ;2) The optimal antigen concentration was 4 mg/L,the optimal antibody dilution was 1:100,the optimal HRP-IgG dilution was 1:20 000.In.120 cases of diseases related to atherosclerosis,positive rate is higher protein antibody against membrane process,up to 25％ ～ 70％,significantly higher than that of normal control group 5％ (P ＜0.01) ; acute coronary syndrome group,the positive rate of 70％,higher than the other groups (P ＜ 0.05),the difference between other subtypes of disease no significant (P ＞ 0.05).Conclusion Successfully expressed the recombinant human moesin protein; Establish the best indirect ELISA of Purified recombinant moesin protein was plated as coating antigen detect moesin antibody ; Moesin antibodies in patients with atherosclerotic disease associated with high detection rate.关键词
膜突蛋白/重组表达载体/抗膜突蛋白抗体/动脉粥样硬化Key words
Moesin/ Expression Vector/ Moesin Antibody/ Atherosclerosis分类
医药卫生引用本文复制引用
毛华,杜峰,胥勇,朱晋坤,文美,刘廷筑..Moesin抗体与动脉粥样硬化相关疾病的关系[J].中国动脉硬化杂志,2013,21(6):549-553,5.
