中国临床药理学与治疗学2013，Vol.18Issue(6)：606-609,4.
硼替佐米对胃癌细胞的抗肿瘤作用及其机制
Cytotoxic activity of bortezomib against gastric cancer and its action mechanism
摘要
Abstract
AIM:To study the cytotoxic activity of bortezomib against gastric cancer and its action mechanism.METHODS:MTT assay was used to detect the cytotoxic activity of bortezomib against gastric cancer SGC-7901 cells.The apoptosis were detected by Annexin VFITC/PI staining and flow cytometry analysis.The protein expressions were determined by Western blot analysis.RESULTS:The IC50 value of bortezomib against SGC-7901 cells was (54.6± 4.1) nmol/L.After treatment with 0,50,and 100 nmol/L bortezomib for 48 h,the percents of apoptosis were (1.8±0.7)％,(26.5±4.6)％,(41.7±5.8) ％,respectively.There was significant difference among the different treatments(P＜0.01).After treatment with 50 and 100 nmol/L bortezomib for 48 h,the eleaved-Parp and cleaved-caspase-3 were detected by Western blot analysis.After treatment with 50 and 100 nmol/L bortezomib for 48 h,the protein expression of Bmi-1 was downregulated.CONCLUSION:Bortezomib shows significant anticancer activity against gastric cancer SGC-7901 cells.The downregulation of Bmi-1 protein may played an important role in its apoptotic pathway.关键词
胃癌/硼替佐米/细胞凋亡/Bmi-1Key words
Gastric cancer/ Bortezomib/ Apoptosis / Bmi-1分类
医药卫生引用本文复制引用
陈辉,瞿丽,黄永刚..硼替佐米对胃癌细胞的抗肿瘤作用及其机制[J].中国临床药理学与治疗学,2013,18(6):606-609,4.
