中国临床药理学杂志2013，Vol.29Issue(6)：466-468,3.
肺部感染患者血清中万古霉素浓度的监测
Monitoring of vancomycin serum concentration of patients with pulmonary infection
徐晓卫 1潘柔和 2胡卢丰1
作者信息
- 1. 温州医学院附属第一医院药剂科,浙江温州 325002
- 2. 温州市药品检验所,浙江温州 325028
- 折叠
摘要
Abstract
Objective To establish a HPLC method for determination of vancomycin in serum and using the method for clinical monitoring the levels of vancomycin of patients with pulmonary infection.Methods HPLC analysis was performed using a Eclipse XDB C18 column (4.6 mm × 250 mm,5 μm).The mobile phase was KH2PO4 (25 mmoL· L-1):methanol (86:14) The flow-rate was 1.0 mL · min-1 and the detection wavelength was 236 nm.The injection volume was 20 μL and the temperature of column was about 40 ℃.Results The concentration range of vancomycin was 5-100 mg · L-1 while the calibration curve showed good linearity (r =0.999 6),and the minimum limit o f quantitation was 5.0 mg · L-1.The coefficient of methods recovery were 94.87％.There lative standard deviations(RSD) of intra-day and inter-day were 6.06％ and 6.27 ％,respectively.More over,the concent ration of vancomycin was lower than 10 mg · L-1 in 26.67％ of those 15 patients with pulmonary infection.Conclusion The method is simple,sensitive,rapid and highly reproducible,and it could be used for detecting the concentration of vancomycin in for pulmonary infection patients.The concentration of vancomycin has a large fluctuation in pulmonary infection and their suitable individual dosage regimen need to be administrated through therapeutic drug monitoring.关键词
高效液相色谱法/药物监测/万古霉素Key words
HPLC/ drug monitoring/ vancomycin分类
医药卫生引用本文复制引用
徐晓卫,潘柔和,胡卢丰..肺部感染患者血清中万古霉素浓度的监测[J].中国临床药理学杂志,2013,29(6):466-468,3.
