中国免疫学杂志2013，Vol.29Issue(6)：570-574,5.DOI:10.3969/j.issn.1000-484X.2013.06.002
大豆甙元联合TRAIL诱导胃癌SGC-7901细胞凋亡的机制研究
Research the mechanism of apoptosis induced by Daidzein combined with TRAIL in SGC-7901 cells
摘要
Abstract
Objective:To investigate the effects of Daidzein combined with TRAIL in gastric adenocarcinoma cell SGC-7901.Methods:SGC-7901 cells were treated with Da or/and TRAIL at different concentration for indicated times.MTT assays were performed to measure the growth inhibitory effect of Da or/and TRAIL.Cell cycle and apoptosis of cells were analyzed using flow cytometry.Transmission electron microscope was introduced to observe the changes of internal morphology and microstrncture of SGC-7901 cells.Western blot was used to examine the changes of Bcl-2,caspase-3,survivin expression.Results:Da combined with TRAIL obviously inhibits the cell growth of SGC-7901 cells in a dose-dependent manner and induces cell cycle arrest in S phase.Typical apoptosis morphology changes were observed after the combined treatment.Bcl-2 and survivin proteins were decreased and the level of caspase-3 was increased upon the combined treatment.Conclusion:Da combined with TRAIL lead to apoptosis of gastric adenocarcinoma cells through induction of cell cycle arrest in S phase,inhibition of Bcl-2 and survivin protein expression,and enhancement of caspase-3 protein level.关键词
大豆甙元(Da)/TRAIL/胃癌SGC-7901细胞/细胞凋亡Key words
Daidzein/TRAIL/Gastric cancer cell SGC-7901/Cell apoptosis分类
医药卫生引用本文复制引用
杜志敏,杨静静,马倩,王天云,李齐印,徐志强,李万里..大豆甙元联合TRAIL诱导胃癌SGC-7901细胞凋亡的机制研究[J].中国免疫学杂志,2013,29(6):570-574,5.基金项目
本文受国家自然科学基金项目(30970055C010202)和河南省新乡医学院高学历人才科研启动资助项目(100179)资助 （30970055C010202）
