中国免疫学杂志2013，Vol.29Issue(6)：615-617,3.DOI:10.3969/j.issn.1000-484X.2013.06.012
甲型H1N1流感病毒亚单位疫苗对小鼠的免疫原性观察
To evaluate the immunogenicity of H1N1 influenza subunit vaccine in mice
摘要
Abstract
Objective:To observe the immunogenicity of domestic H1N1 influenza subunit vaccine in mice.Methods:The H1N1 influenza subunit vaccines with various hemagglutinin contents (20,30,40 μg/ml),three batches for each,were used for immunization of mice.Mice were divided various groups,10 for each,and were injected ip.with 1 and 2 doses of influenza subunit vaccines with various hemagglutinin contents respectively,0.5 ml for each,using those unimmunized as a control group.The interval between doses was one week.Serum samples were collected 21 d after the first dose and determined for hemagglutination inhibition (HI) and neutralizing antibody titers.Results:The HI and neutralizing antibody titers of mice immunized with 1 and 2 doses of H1N1 influenza subunit vaccine (hemagglutinin contents of 20 μg/ml) were less than 1∶40 and 1∶200 respectively.However,the HI antibody titers of mice immunized with 1 and 2 doses of H1N1 influenza subunit vaccine (hemagglutinin contents of 30,40 μg/ml) were more than 1 ∶ 40,and nuetralizing antibody titers were more than 1 ∶ 200.Neither HI nor neutralizing antibody titers showed significant difference in the mice immunized with 1 and 2 doses of H1N1 influenza subunit vaccine (hemagglutinin contents of 30,40 μg/ml).Conclusion:The immune effects of one dose of H1N1 influenza subunit vaccine with hemagglutinin contents of 30 μg/ml are satisfactory.关键词
流感病毒亚单位疫苗/血凝素/小鼠/免疫原性Key words
H1N1 Influenza subunit vaccine/Hemagglutinin/Mice/Immunogenicity分类
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刘英,郭世杰,闫昆明,严超英,王明惠,王飞宇,傅连弟,韩军..甲型H1N1流感病毒亚单位疫苗对小鼠的免疫原性观察[J].中国免疫学杂志,2013,29(6):615-617,3.基金项目
本文为长春市社会发展科技计划资助项目(10SF03) （10SF03）
