中国免疫学杂志2013，Vol.29Issue(6)：618-621,624,5.DOI:10.3969/j.issn.1000-484X.2013.06.013
不同类型HBV感染者外周血Treg/Th17细胞的变化及意义
Changes and clinical significances of Treg and Th17 cells in the peripheral blood of patients with different types of hepatitis B virus infection
王萍 1胥冰 1张丽君1
作者信息
- 1. 陕西中医学院免疫教研室,咸阳712046
- 折叠
摘要
Abstract
Objective:To explore the changes and clinical significances of CD4 + Foxp3 + Treg/Th17 cells in the peripheral blood of patients with different types of hepatitis B virus (HBV) infection.Methods:15 patients with acute hepatitis B (AHB),40 patients with chronic hepatitis B (CHB),40 patients with asymptomatic HBV carriers (AsC) and 30 healthy individuals were recruited.The percentage of CD4 + Foxp3 + Treg/Th17 cells were assessed by flow cytometry and the mRNA levels of Foxp3/RORγt were detected by real-time quantitative reverse transcription polymerase chain reaction (RT-PCR).The plasma levels of TGF-31/IL-17 were measured by enzyme linked immunosorbent assay (ELISA).Results:The patients with AHB,compared with normal controls,had no significantly differences in the percentage of CD4 + Foxp3 + Treg cells,the mRNA expression of Foxp3 and the plasma levels of TGF-β1 (P ＞ 0.05).The percentage of Th17 cells,the mRNA expression of RORγt and the plasma levels of IL-17 increased significantly in patients with AHB than in normal controls (P ＜ 0.05).The percentage of CD4 + Foxp3 + Treg/Th17 cells,the mRNA expression of Foxp3/RORγt and the plasma levels of TGF-β1/IL-17 increased significantly in patients with CHB than in normal controls (P ＜0.05).The patients with AsC,compared with normal controls,had no significantly differences in the percentage of CD4 + Foxp3 +Treg/Th17 cells,the mRNA expression of Foxp3/RORγt and the plasma levels of TGF-31/IL-17 (P ＞0.05).Conclusion:The imbalance of Treg/Th17 cells may exist in the peripheral blood of patients with different types of HBV infection.Treg/Th17 cells may be associated with the states of HBV infection.关键词
Treg细胞/Th17细胞/乙型肝炎/类型Key words
Treg cell/Th17 cell/Hepatitis B virus/Type分类
医药卫生引用本文复制引用
王萍,胥冰,张丽君..不同类型HBV感染者外周血Treg/Th17细胞的变化及意义[J].中国免疫学杂志,2013,29(6):618-621,624,5.
