中国农业科学2013，Vol.46Issue(4)：657-667,11.DOI:10.3864/j.issn.0578-1752.2013.04.001
转Hpa110-42小麦分子鉴定及赤霉病抗性功能评价
The Molecular Identification of Transgenic Hpa110-42 Wheat and Resistance Evaluation on Fusarium Head Blight
摘要
Abstract
[Objective] This study aims to screen Fusarium head blight (FHB) resistance plants in Hpa110-42 transgenic wheat plants and provide materials for FHB resistance breeding of wheat.[Method] T2 transgenic wheat plants were identified by PCR,Southem blot and RT-PCR,assuring whether the foreign Hpa110-42 gene was integrated and expressed in transgenic wheat plants.The resistance to FHB of transgenic wheat plants was evaluated by a dripping method to single flower,and its resistance physiology was studied.[Result] PCR,Southern blotting and RT-PCR analyses have confirmed successful integration of the foreign Hpa110-42 gene into the genome of the Yangmai 158 with 1 to 3 copies and stable inheritance and expression.Disease bioassays of transgenic plants revealed that the average percentage of diseased spikelets of transgenic lines T2-17,T2-15,T2-68,T2-44 and T2-36 were highly significantly lower than YM158.Compared with Sumai 3,the average percentage of diseased spikelets of all transgenic lines was highly significantly higher than Sumai 3 excepting line T2-17.In the transgenic lines,the average percentage of diseased spikelets of line T2-17 was significantly lower than other lines,in which T2-36,T2-11 and T2-20 have reached highly significant difference level.It is showed by physiological analysis that the activities of PAL,chitinase and β-1,3-glucanase in the high resistance transgenic plants increased faster than Yangmai 158 after inoculation.The soluble protein content in all plants had a downward trend,while its content in high resistance plants is consistently higher than others.Moreover,β-l,3-glucanase activity and soluble protein content were negatively correlated with resistance grade of FHB.[Conclusion] The foreign Hpal10-42 gene was stably inherited,normally expressed and positively participated in the regulation of FHB resistance,and Hpa110-42 transgenic wheat planns with resistance to FHB were obtained.关键词
Hpa110-42/转基因小麦/基因表达/赤霉病/抗性生理Key words
Hpa110-42/ transgenic wheat plants/ gene expression/ Fusarium head blight/ resistance physiology引用本文复制引用
杨敏,秦保平,刘昌来,蔡洪生,王振林,梁元存,尹燕枰..转Hpa110-42小麦分子鉴定及赤霉病抗性功能评价[J].中国农业科学,2013,46(4):657-667,11.基金项目
国家转基因生物新品种培育重大专项(2009ZX08002-004B)、国家公益性行业(农业)科研专项(201203029)、国家重点基础研究计划(973计划)项目(2009CB118602) （2009ZX08002-004B）
