中国农业科学2013，Vol.46Issue(4)：810-818,9.DOI:10.3864/j.issn.0578-1752.2013.04.015
猕猴桃果实贮藏期主要真菌病害的rDNA-ITS鉴定及序列分析
Molecular Identification and rDNA-ITS Sequence Analysis of Fungal Pathogens in Kiwifruit During Storage
摘要
Abstract
[Objective] The fungal pathogens of ‘China optimal’,‘Haiwode’ and ‘Qinmei’ kiwi fruit that were harvested in Zhouzhi County,Shaanxi Province,were isolated during storage and identified by rDNA-ITS sequences analysis.[Method] With rDNA-ITS analysis and morphological characterization,the species of Fungal pathogens were differentiated and identified.By means of the genetic relationship analysis and multiple comparison analysis of ITS sequences of fungal pathogens,the diversity of intra-species in the pathogens were investigated.[Result] Five strains were identified,e.g.Penicillium,Trichoderma,Alternaria,Mucor and Simplicillium.Penicillium was the dominant species,accounting for 63.3％ of the tested strains.And Trichoderma was the second dominant species,accounting for 26.7％.There were little differences in ITS sequences among Trichoderm,approval ratings were 99％.But the differences of ITS sequences between the Penicillium were significant.Such as Penicillium sp.,Penicillium purpurogenum,Penicillium chrysogenum,Penicillium paneum and Penicillium commune.[Conclusion] Penicillium are mainly fungal pathogens of ‘China optimal’,‘Haiwode’ and ‘Qinmei’ kiwifruit that were harvested in Zhouzhi County,Shaanxi Province.The differences of rDNA-ITS sequences between Penicillium are obvious.So,rDNA-ITS sequence between ourtra-species and intra-species have a certain reference value.关键词
猕猴桃/真菌/鉴定/rDNA-ITS序列分析Key words
kiwifruit/ fungal/ identification/ rDNA-ITS sequence analysis引用本文复制引用
段爱莉,雷玉山,孙翔宇,高贵田,赵金梅,谷留杰..猕猴桃果实贮藏期主要真菌病害的rDNA-ITS鉴定及序列分析[J].中国农业科学,2013,46(4):810-818,9.基金项目
陕西省"13115"科技创新工程重大科技专项(2009ZDKG-05)、陕西省科技统筹创新工程计划项目(2012KTJD03-05)、猕猴桃质量安全技术研究与示范(771845)、西安市科技项目:果树等品种选育、快繁、贮藏及病虫害防治技术研究与应用-猕猴桃贮藏期灰霉病、青霉病防治关键技术研究(NC1116(2)) （2009ZDKG-05）
