中国农业科学2013，Vol.46Issue(4)：859-870,12.DOI:10.3864/j.issn.0578-1752.2013.04.020
不同气候环境中团棵期烟草叶片蛋白质组学分析
Proteomic Analysis of Tobacco Rosette Stage Leaves Under Different Climatic Conditions
摘要
Abstract
[Objective] The effects of different climatic conditions on the molecular developmental mechanism of rosette stage tobacco were studied.[Method] Under the same conditions of soil,cultivation and management,flue-cured tobacco cuItivar Yun 87 was pot-planted in the farm of Yunnan Agricultural University in Kunming and Jinzhuang of Yulong county in Lijiang,respectively.When tobacco developed into the rosette stage,the agronomic traits,the SPAD values and the net photosynthetic rate were determined,simultaneously,total proteins of the tobacco leaves were separated by two-dimensional electrophoresis(2-DE) and the more than 2 times differential expression proteins were analyzed through MALDI-TOF-MS.[Result] Compared with the Kunming experimental site,the tobaccos planted in Jingzhuang had the higher SPAD value,net-photosynthetic rate and the speeding of developmental process resulted in the increase of the plant height,length of inter stem nodes,stem circumference,leaf width as well as leaf area.The proteomic analysis shown that the leaves of tobaccos planted in both experimental sites had 39 protein spots with more than 2 times differential expression,in which 33 were identified and annotated.Among the annotated ones,compared with Kunming site,tobaccos of Jingzhang site had 3 (9.09％) specifically expressed,7 (21.21％) up-regulated,19 (57.57％)down-regulated proteins,whilst the tobaccos planted in Kunming had 4 (12.12％) specific proteins.The functional classification of the differentially expressed proteins belonged to tobacco metabolism of photosynthesis,defense or (and) stress tolerance,chloroplast protein synthesis,mineral metabolism and so on.[Conclusion] Compared with rosette stage tobaccos of Kunming,the counterparts of Jingzhuang had higher SPAD value,net-photosynthetic rate,and larger agronomic traits,such as plant height,length of inter stem nodes,stem circumference,leaf width as well as leaf area,and those performance was consistent to that the Jingzhuang tobaccos accumulated proteins related to higher photosynthetic efficiency,whilst the Kunming tobaccos enriched the proteins for improving stress tolerance,chloroplast protein biosynthesis and mineral metabolism as well.The results preliminarily illuminated the molecular mechanism of Lijiang tobacco development with characteristics which contains much more total sugars and reduction sugars,while less total nitrogen and nicotine.关键词
烟草/团棵期/农艺性状/蛋白质组/气候环境Key words
Nicotiana tabacum/ rosette stage/ agronomic traits/ proteome/ climatic conditions引用本文复制引用
蔡永占,周普雄,李佛琳,赵昶灵,林春,杨焕文,毛自朝..不同气候环境中团棵期烟草叶片蛋白质组学分析[J].中国农业科学,2013,46(4):859-870,12.基金项目
中国烟草总公司重大科技专项(110200902034,110201101003 (TS-03))、云南省烟草公司科技项目(09YN022,2011YN03) （110200902034,110201101003 (TS-03）
