中国全科医学2013，Vol.16Issue(12)：1339-1341,3.DOI:10.3969/j.issn.1007-9572.2013.04.082
不同乳腺癌细胞冻融抗原负载树突细胞的抑瘤功能研究
Research of the Antitumor Functions of Dendritic Cells Pulsed with Various Types of Breast Cancer Cell Lysate Antigens
曹天泽 1高维实 1郭慧军 1马虎林 1施晓辉1
作者信息
- 1. 010010,内蒙古呼和浩特市,内蒙古自治区人民医院肿瘤外科
- 折叠
摘要
Abstract
Objective To investigate the antitumor functions of dendritic cells ( DCs ) pulsed with various types of breast cancer cell lysate antigens. Methods Purified breast cancer cells ( group A ) and breast cancer tissues ( group B ) from patients underwent breast cancer surgery in the surgical oncology of Inner Mongolia Autonomous Region Hospital, and breast cancer cell line SKBR - 3 ( group C ) purchased from Peking Union Medical College were involved into the study. Tumor lysate antigens were prepared and DC vaccines were obtained. The surface molecule expression of DCs and function were tested by flow cytometer and the T lymphocyte proliferation test. Results After seven days inducement, the CD80, CD83 , CD86 and HLA - DR between the three groups' DC showed statistically significant differences ( P <0. 05 ) . Compared with group B, the group A and group C both showed statistically significant differences ( P < 0. 05 ) . The group A and group C showed no statistically significant difference ( P > 0. 05 ) .The CPM value of group A ( 13 047 ± 215 ), group B ( 4 964 ± 148 ) and group C ( 5 082 ± 176 ) showed statistically significant differences ( F = 5 200. 7159, P =0. 0000 ) . Compared with group A, the group B and group C both showed statistically significant differences ( q = 125. 8166, 123. 9803 , P <0. 01 ) . The group B and group C showed no statistically significant difference ( q = 1. 8363 , P > 0. 05 ) . Conclusion DCs pulsed with purified autologous tumor cell lysate antigens can mediate remarkable immune response of T lymphocyte.关键词
乳腺肿瘤/树突细胞/疫苗Key words
Breast neoplasms/ Dendritic cells/ Vaccine分类
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曹天泽,高维实,郭慧军,马虎林,施晓辉..不同乳腺癌细胞冻融抗原负载树突细胞的抑瘤功能研究[J].中国全科医学,2013,16(12):1339-1341,3.
