中国人兽共患病学报2013，Vol.29Issue(6)：533-536,4.DOI:10.3969/cjz.j.issn.1002-2694.2013.06.002
天蚕素多肽分子对大肠埃希菌的抑制作用
Inhibition of Escherichia coli growth by cecropin A
王炜 1任伟宏1
作者信息
- 1. 河南中医学院第一附属医院,郑州,450000
- 折叠
摘要
Abstract
The aim of this study is to express cecropin A from the Musca domestica and identify its activity in vitro . Housefly cecropin mature peptide gene was cloned to construct pET 32a-mc plasmid. The plasmid pET32a-mc was then transformed into BL21 (DE3) to express the recombinant protein after the induction of IPTG . The Trx&MC fusion protein was purified and the Trx&MC fusion protein was digested by pepsin . After that the antibacterial activity of MC was detected by liquid growth inhibition . Results showed that the MC molecule had the same activity in vitro on standard strains and multiple drug-resistant strains with E. coli, which concluded that MC molecules had inhibitory on multiple drug-resistant (MDR) E. coli strain and could be as an alternative to traditional antibiotics .关键词
天蚕素/伴侣分子/融合表达/抑菌试验/多重耐药菌Key words
Musca domestica/ cecropin/ molecular partner / fusion expression / multiple drug resistant分类
医药卫生引用本文复制引用
王炜,任伟宏..天蚕素多肽分子对大肠埃希菌的抑制作用[J].中国人兽共患病学报,2013,29(6):533-536,4.
