中国人兽共患病学报2013，Vol.29Issue(6)：562-565,569,5.DOI:10.3969/cjz.j.issn.1002-2694.2013.06.008
狂犬病毒核蛋白时间分辨免疫荧光分析试剂盒研制
Preparation of time-resolved fluoroimmunoassay kit for rabies virus nucleoprotein detection
摘要
Abstract
The aim of this study is to develop the kit of time-resolved fluoroimmunoassay (TRFIA ) for Rabies Virus (RV), nucleoprotein (NP) . The RV NP TRFIA Kit was processed by double antibody sandwich method and assessed by the given indicators . The results of this kit possessed high accuracy , wide linear range from 5 to 2 500 mEU/mL , high sensitivity with 1 .018 mEU/mL , and good precision . The coefficient variations of the intra- and inter-assay were 2 .7% to 9 .3% and 3 .5% to 12 .2% , respectively . The correlation coefficient was found with 0 .85 by detecting 15 samples of vaccines between this developed kit and domestic ELISA kit which is used popularly nowadays . As well, the stability tests showed that reagents could be stable for six months at 4 ℃ , 7 days at 37 ℃ , respectively . Our investigation showed that the indicators of the developed kit met the requirements of clinical testing and the developed kit could be used for the tests in clinical detection .关键词
狂犬病毒/核蛋白/定量检测/双抗夹心时间分辨免疫荧光分析Key words
rabies virus / nucleoprotein / quantitative assay / double-antibody sandwiched time-resolved fluoroimmunoassay分类
医药卫生引用本文复制引用
黄黉,林冠峰,陈少琅,刘天才,李明,吴英松..狂犬病毒核蛋白时间分辨免疫荧光分析试剂盒研制[J].中国人兽共患病学报,2013,29(6):562-565,569,5.基金项目
艾滋病和病毒性肝炎等重大传染病防治(No.2009ZX10004-706)资助 （No.2009ZX10004-706）
