中国人兽共患病学报2013，Vol.29Issue(6)：566-569,4.DOI:10.3969/cjz.j.issn.1002-2694.2013.06.009
浙江省甲型H1N1流感病毒血凝素基因序列分析
Sequence analysis of hemagglutinin gene of pandemic influenza A (H1N1) viruses in Zhejiang Province
摘要
Abstract
The aim was to analyze the evolutionary characteristics of hemagglutinin gene of pandemic influenza A (H1N1) virus isolated from Zhejiang Province during 2009 and 2011 . Viral RNA was extracted from 8 influenza A (H1N1) virus strains . Hemagglutinin gene was amplified by RT-PCR and the ORF sequence was joined together using sequencing data . Hemagglutinin genes from 20 representative domestic and overseas strains during 2009 to 2012 were downloaded from GenBank database for analysis . All the sequences were used for construction of phylogenetic tree by MEGA software . The 1 701 bp ORF sequences of hemagglutinin gene were obtained by analyzing sequencing results of the 8 strains . After alignment, high homolo-gy were observed among all the HA genes originated all over the world . The homology between 3 strains from Zhejiang Province during 2009 and 2010 and the representative strain of A/California/04/2009 (H1N1) from North America was as high as 99.4% -99.5% . The homology between 5 strains from Zhejiang Province isolated in 2011 and the A/California/04/2009 (H1N1) was 98 .5% -99 .1% . A D222G mutation in hemagglutinin protein was observed in the A /Zhejiang-Yiwu/11/2009 (H1N1) strain isolated from a patient with severe influenza A (H1N1) infection . Although more mutations were accumulated in HA genes in the strains from Zhejiang Province in 2011 comparing with strains from early stage of the pandemic , high homology of more than 98 .5% was observed in all the HA genes . A D222G mutation in HA might be related to the severe illness .关键词
甲型H1Nl流感病毒/血凝素/序列分析/种系发生树Key words
pandemic H1N1 influenza virus / hemagglutinin / sequence analysis / phylogenetic tree分类
医药卫生引用本文复制引用
李敏红,周敏,茅海燕,张严峻,卢亦愚,陈寅..浙江省甲型H1N1流感病毒血凝素基因序列分析[J].中国人兽共患病学报,2013,29(6):566-569,4.基金项目
浙江省自然科学基金(No.Y207819)和浙江省医药卫生科学研究基金(No.2010KYB031)资助 （No.Y207819）
