中国人兽共患病学报2013，Vol.29Issue(6)：601-604,4.DOI:10.3969/cjz.j.issn.1002-2694.2013.06.015
狂犬病病毒N、G两种蛋白在间接ELISA方法中的应用比较
Application and comparison of rabies virus N and G proteins in indirect ELISA
摘要
Abstract
The aim of this study is to evaluate the effect of detection for Rabies virus antibody level with recombinant G and N proteins . After comparing of the expression quantity of Rabies virus G and N proteins in pET -32a (+ ) and pGEX-6P-l expression vectors , we chose the recombinant proteins with high expression quantity to purify with the carbonate coating buffer (pH = 9.6). The Rabies virus antibody level in the 93 serums samples were detected by the indirect ELISA using purified pGEX-G and pGEX-N protein as coating antigen respectively . The results showed that the recombinant proteins pGEX-G and pGEX-N with high expression quantity were highly purified with the carbonate coating buffer . The result of the indirect ELISA were positive for all the serum samples , indicated that all of the tested dogs produced antibodies after vaccination . But there are some differences , the detection value of ELISA with pGEX-G were higher than pGEX-N in 56 serum samples , for other serum samples, we get the opposite result . The results indicated that anti-G antibody and anti-N antibody were produced at different times in the immune canines . Which kind of antibody appear more early , remains to be further studied .关键词
狂犬病/N蛋白/G蛋白/pET-32a(+)/pGEX-6P-1/蛋白纯化/抗体检测Key words
rabies virus / N protein / G protein / pET-32a (+ ) / pGEX-6P-l/ protein purification / antibody detection分类
医药卫生引用本文复制引用
王净,史利军,李刚,孙全文,吴淑琴..狂犬病病毒N、G两种蛋白在间接ELISA方法中的应用比较[J].中国人兽共患病学报,2013,29(6):601-604,4.基金项目
国家科技支撑计划(No.2013BAD12B05),国际合作项目(No.D32025/17453),国家自然科学基金(No.31172342),国家公益性行业科研专项(No.200903037),河北省畜牧兽医局科技项目(No.2012-3-03)联合资助 （No.2013BAD12B05）
