中国输血杂志2013，Vol.26Issue(5)：426-429,4.
ABCA1 DNA甲基化水平与AS及血脂的关系
The relationship among ABCA1 DNA methylation and atherosclerosis and blood lipids
摘要
Abstract
Objective To analysiz the relationship among DNA methylation level of ATP binding cassette transporter A1 (ABCA1) and atherosclerosis occurrence,development and changes of blood lipids.Methods The atherosclerosis patients were confirmed by carotid artery Doppler ultrasound and the serum lipids were measured by ADVIA 2400 Automatic Biochemistry Analyzer.Thirty cases serum of atherosclerosis patients (AS group) and healthy people (control group) were collected,and then DNA was extracted.Fluorescence quantitative PCR were used to detect the mRNA expression of ABCA1.The methylation status changes of ABCA1 were detected by nMS-PCR.And the correlation of DNA methylation levels and blood lipid level was analyzed.Results Serum lipids were detected by automatic biochemical analyzer,and compared with normal group,the TG content of AS group increased 2.17 times (P ＜ 0.01),CHOL levels increased 1.21 times (P＜0.05),HDL increased 0.83 folds,LDL increased 1.18 times (P ＜0.05).Besids,compared with the normal control group,the ABCA1 mRNA levels of AS patients decreased by 63.3％ (P ＜0.01),and the ABCA1 DNA methylation level in AS group increased by 1.38 times (P ＜0.01).And the correction analysis showed that lipid levels and ABCA1 DNA methylation levels were positively correlated (r =0.489 8,P ＜ 0.01).Condusion The ABCA1 DNA hypermethylation is closely related to the occurrence and development of atherosclerosis.关键词
动脉粥样硬化/三磷酸腺苷结合盒运转体/DNA甲基化/血脂/mRNA表达量Key words
atherosclerosis/ ATP binding cassette transporter A1 / DNA methylation/ blood lipids/ mRNA expression分类
医药卫生引用本文复制引用
韩学波,张慧萍,马胜超,曹成建,杨程,王艳华,孙炜炜,姜怡邓..ABCA1 DNA甲基化水平与AS及血脂的关系[J].中国输血杂志,2013,26(5):426-429,4.基金项目
国家自然科学基金地区项目(81260105)、宁夏自然科学基金(NZ1195)、宁夏回族自治区科技厅科技攻关项目(20100820)、宁夏回族自治区教育厅重点项目(宁教高[2010]297)、宁夏回族自治区卫生厅项目(2011027) （81260105）
