中国实验动物学报2013，Vol.21Issue(3)：27-30,69,后插5,6.DOI:10.3969/j.issn.1005-4847.2013.03.006
CT灌注成像与大鼠C6胶质瘤CD105表达的相关性
Correlation between perfusion CT imaging and CD105 expression in rat brain C6 gliomas
摘要
Abstract
Objective To investigate the features of CT perfusion imaging,and analyze the correlation between CT perfusion paremeters and angiogenesis in rat C6 gliomas.Methods In a total of 20 male SD rats,weighting 260-280 g,were divided into the glioma group (n =10) and control group (n =10) at random.Rats in the control group were injected 10 μL normal saline into the fight caudate nucleus of the brain using a stereotaxic apparatus.Rats in the glioma group were implanted C6 glioma cells at the fight caudate nucleus of brain using the same stereotaxic apparatus to establish a rat brain glioma model.After three weeks,perfusion CT was performed in the rats of both groups.Perfusion CT values of the right nucleus caudatus (CBV,CBF,PS and MTT) were measured.The rats were killed and their brains were removed within 24 hours after perfusion CT,and the tissue samples of nucleus caudatus were examined histologically using HE and immunohistochemical staining for FVⅢ and CD105.The differences between the number of FVIII-positive vessels within the gliomas and that of normal brain tissues in the control rats were determined using independent samples t test.Pearson correlation coefficients were used to evaluate the relationships between perfusion CT parameters (CBV,CBF,PS and MTT) and immunohistochemical parameters (FVⅢ-MVD and CD105-MVD).Results All of the rat brain gliomas at 3 weeks after tumor cells implantion showed to be a significantly enhanced mass on contrast enhancement CT scan.The CBV,CBF,PS and MTT values (mean ± SD) of the control group were (10.28 ± 4.01) mL/100 g,(304.95 ± 88.77) mL/100 g.min,(0.26 ±0.34) mL/100 g.min,and (1.48 ±0.07) s,respectively.The CBV,CBF,PS and MTT values (mean ± SD) of the gliomas at right caudate nucleus area were (17.35 ± 6.73) mL/100 g,(508.66 ± 158.88) mL/min.100 g,(13.92 ± 8.96) mL/min.100 g,and (1.79 ±0.44) s,respectively.The CBV,CBF and PS values in the glioma group were significantly higher than those in the control group.FVIII-MVD and CD10S-MVD in the glioma group were (34.7 vessels per field ± 7.13) and (16.6 vessels per field ± 4.12),respectively.Significant correlations were observed between FVⅢMVD,CD105-MVD and CBV,PS in the glioma group.CBF correlated well with CD105-MVD,while CBF was not significantly correlated with FVⅢ-MVD in the glioma group.MTT results did not show significant correlation with MVD in the glioma group.Conclusion Perfusion CT parameters (CBV,CBF and PS) are significantly correlated with CD105-MVD expression in the rat glioma,and perfusion CT imaging is helpful in research of glioma angiogenesis.关键词
胶质瘤/血管生成/体层摄影术,X线计算机/灌注/CD105Key words
Glioma/ Angiogenesis / Tomography, X-ray computed / Perfusion / CD105分类
生物科学引用本文复制引用
张家文,冯晓源,姚振威,刘含秋,陆娜,邱龙华,杨艳梅,黎元..CT灌注成像与大鼠C6胶质瘤CD105表达的相关性[J].中国实验动物学报,2013,21(3):27-30,69,后插5,6.基金项目
国家自然科学基金(项目编号:30970805) （项目编号:30970805）
上海市卫生局局级科研项目基金(项目编号:20114186). （项目编号:20114186）
