中国实验动物学报2013，Vol.21Issue(3)：85-88,封4,5.DOI:10.3969/j.issn.1005-4847.2013.03.018
氟对大鼠肝组织细胞凋亡与DNA损伤的影响
Effects of fluoride on apoptosis and DNA damage in rat hepatocytes
刘茂林 1宋国华 1高继萍 1樊林花 1王裕1
作者信息
- 1. 山西医科大学实验动物中心,太原030001
- 折叠
摘要
Abstract
Objective To explore the cell apoptosis and DNA damage induced by fluoride in the rat liver.Methods Forty-eight male SD rats were randomly divided into control group,low-dose fluorine group,middle-dose fluorine group and high-dose fluorine group,each group of 12 rats.The rats were administered with 0,50,100,and 200 mg/ L sodium fluoride for 120 d.The dental changes were observed by visual inspection.The urinary fluoride was determined by fluoride-ion selective electrode analysis.The pathological changes of liver tissues were observed using HE staining.The DNA damage was tested by comet assay,and the apoptosis rate of hepatocytes was measured by flow cytometry.Results The contents of urinary fluoride were (23.52 ± 2.91),(30.16 ± 4.78) and (61.23 ± 3.98)mg/L in the three fluoride groups,respectively,significantly higher than that of the control group (0.07 ±0.02 mg/L) (P ＜0.01).The rat hepatocytes of different fluoride dose groups showed varying degrees of cell swelling and pathological changes.The tailing rate and tail length of rat hepatocytes in the fluoride groups were significantly higher than that of the control group,with statistically significant differences.The tailing rate and tail length of hepatocytes were increasing with the increase of fluoride doses.The cell apoptosis rates in the fluoride groups were significantly higher than that of the control group,and the apoptosis rates in the middle-dose fluorine group and high-dose fluorine group were significantly higher than that of the low-dose fluorine group (P ＜ 0.01).Conclusions Fluoride exposure can cause DNA damage and apoptosis in hepatocytes,and there exists a significant positive correlation between DNA damages and apoptosis and the fluoride concentrations in a certain range.关键词
氟/大鼠/肝/细胞凋亡/DNA损伤Key words
Fluoride/ Rats/ Liver/ Apoptosis/ DNA Damage分类
生物科学引用本文复制引用
刘茂林,宋国华,高继萍,樊林花,王裕..氟对大鼠肝组织细胞凋亡与DNA损伤的影响[J].中国实验动物学报,2013,21(3):85-88,封4,5.
