中国比较医学杂志2013，Vol.23Issue(6)：6-11,封2,7.DOI:10.3969.j.issn.1671.7856.2013.006.002
MicroRNA-424通过增加转录因子的表达减轻小鼠缺血性脑损伤
MicroRNA-424 increases the expression of transcriptional factors and reduces the ischemic brain injury in the mouse
摘要
Abstract
Objective To investigate the effect of microRNA-424 (miR-424) on neuronal apoptosis after cerebral ischemia in mice.Methods The lentiviral mediated overexpression of miR-424 (109 U/mL,8 μL) was injected into the lateral ventricle in mice for 7 d,then the middle carotid artery occlusion (MCAO) model was established,and the mice were divided into 4 groups:sham group,sham + miR-424 group,MCAO model group and MCAO + miR-424 group (n =6).At 8 h after MCAO,the ischemic brain tissue was removed and paraffin sections were TUNEL-stained to observe the neuronal apoptosis,and the protein levels of transcription factor PU.1,hypoxia inducible factor-1 α (HIF-1 α),apoptosisrelated proteins p53 were detected by Western blot.Results The TUNEL immunofluorescence observation revealed that miR-424 reduced neuronal apoptosis at 8 h after cerebral ischemia in the mice.The Western blot showed that before ischemia and at 8 h after ischemia,miR-424 increased the levels of PU.1,HIF-1α and apoptotic protein p53 in the normal and MCAO model brain tissues,exhibiting the same effect on the expression of those three proteins.Conclusions miR-424 can reduce neuronal apoptosis after cerebral ischemia partly through increasing the expression of transcription factor PU.1 and HIF-1α,as well as apoptosis-related proteins p53 in the brain tissue of mice.关键词
microRNA-424/脑缺血/低氧诱导因子-1aKey words
MicroRNA-424/ Cerebral ischemia/ Hif-1a/ Mice分类
医药卫生引用本文复制引用
赵海苹,王荣亮,高志,罗玉敏..MicroRNA-424通过增加转录因子的表达减轻小鼠缺血性脑损伤[J].中国比较医学杂志,2013,23(6):6-11,封2,7.基金项目
国家自然科学基金项目(81071058,30770743,81271461,81201028). （81071058,30770743,81271461,81201028）
