中国比较医学杂志2013，Vol.23Issue(6)：38-43,后插5-后插7,9.DOI:10.3969.j.issn.1671.7856.2013.006.009
转染MIP-1α和B7-1基因增强小鼠的抗淋巴瘤效应
Inhibitory effect of MIP-1α and B7-1 gene transfection on mouse lymphoma
摘要
Abstract
Objective To study the antitumor effect of MIP-1α and B7-1 gene transfection on mouse lymphoma.Methods Mouse lymphoma EL-4 cells were transfected by lentivirus-mediated mouse MIP-1α and B7-1 gene vectors.MIP-1α and B7-1 mRNA were identified by RT-PCR,and MIP-1α and B7-1 proteins were determined by Western blot.The transfected EL-4 cells were injected into the right axilla in mice to induce tumor growth.The transfected EL-4 cells were inactivated and injected into tumor-bearing mice to observe their antitumor effect.Results RT-PCR assay showed expressions of MIP-1 α and B7-1 mRNA in the EL-4/MIP-1α + B7-1 cells,and Western blot revealed expression of MIP-1 α and B7-1 proteins.The tumor formation was retarded,tumor formation rate was decreased,and the tumor was smaller in the MIP-1α group and B7-1 group than that in the contro1 group,and no tumor was formed in the MIP-1α + B7-1 group.The mean tumor volume,weight and organ metastasis rate of the MIP-1 α and B7-1 groups were significantly lower than that of the control group (P ＜0.05),while the mean tumor volume and weight of the MIP-1α + B7-1 group were significantly lower than that of the MIP-1 α group and B7-1 group (P ＜ 0.05).In addition,the mean survival time of the MIP-1 α + B7-1 group was significantly longer than that in the single groups and the control group (P ＜ O.05).Conclusions Lentivirusmediated mouse MIP-1 α and B7-1 gene transfection can effectively enhance the anti-lymphoma effect on tumor-beating mice,and can significantly prolong the mean survival time of tumor-bearing mice.It may provide a new idea for gene therapy of lymphoma in future.关键词
慢病毒载体/EL-4细胞/MIP-1α基因/B7-1基因/淋巴瘤Key words
Lentivirus vector/ Lymphoma cells / MIP-1 α gene / B7-1 gene / Lymphoma / Mouse分类
医药卫生引用本文复制引用
刘伟,余英豪..转染MIP-1α和B7-1基因增强小鼠的抗淋巴瘤效应[J].中国比较医学杂志,2013,23(6):38-43,后插5-后插7,9.基金项目
南京军区医学科学技术研究"十一五"计划课题(编号:07Z034) （编号:07Z034）
福建省自然科学基金(编号:2010J01221)资助. （编号:2010J01221）
