中国循证儿科杂志2013，Vol.8Issue(3)：224-227,4.DOI:10.3969/j.issn.1673-5501.2013.03.014
热休克蛋白70-2和肿瘤坏死因子α基因多态性与儿童过敏性紫癜相关性研究
Association of heat shock protein 70-2 and tumor necrosis factor-α gene polymorphisms with Henoch schonlein-purpura in children
摘要
Abstract
Objective Recently some research has shown that heat shock protein 70-2( HSP70-2 ) gene and tumour necrosis factor-α( TNF-α ) gene polymorphisms may be associated with susceptibility to autoimmune diseases. This study aimed at exploring the correlation between HSP70-2 gene polymorphism( + 1267 A/G )or TNF-α gene polymorphism ( - 308G/A ) and Henoch-scholein purpura( HSP ) in children. Methods 205 children with HSP and 53 healthy controls were enrolled in this study. HSP70-2 genotypes were analyzed by the polymerase chain reaction-restriction fragment length polymorphism technique. Results The frequency of GG genotype was significantly different between HSP group and healthy control group(χ2 =4. 764, P <0. 05 ). The genotype frequency of A and G alleles between HSP group and healthy control group had no statistical significance(χ2 =2. 689,P > 0.05 ). The frequency of genotypes of HSP70-2 gene( + 1267A/G ) between HSP without kidney damage group and HSP with nephritis group showed no significant statistical difference ( χ2 =0. 063 , P > 0. 05 ). The frequency of AA genotype was significantly different between HSP group and healthy control group(χ2 =6. 447, P <0. 05 ). The genotype frequency of A alleles were 24. 4% and 12. 3% in HSP group and healthy control group(χ2 =7. 241 , P <0. 05 ). The frequency of genotypes of TNF-α gene( -308 G/A ) between HSP without kidney damage group and HSP with nephritis group showed no significant statistical difference( χ2 -4. 474 , P > 0.05 ). The genotype frequency of A or G alleles between HSP without kidney damage group and HSP with nephritis group had no statistical significance(χ2 =2. 689,P >0.05 ). Conclusions GG homozygous of HSP70-2 gene ( + 1267A/G ) and AA homozygous of TNF-α gene ( - 308G/A ) might be a genetic predisposing predictor for HSP in children. There was no significant association between GG homozygous and nephritis in HSP.关键词
热休克蛋白70-2/肿瘤坏死因子α/基因多态性/过敏性紫癜Key words
Heat shock protein 70-2/ Tumour necrosis factor-a/ Gene polymorphisms/ Henoch schonlein- purpura引用本文复制引用
管皖珍,杨小凤,袁杨刚,苏敏,丁桂霞,黄松明,张爱华..热休克蛋白70-2和肿瘤坏死因子α基因多态性与儿童过敏性紫癜相关性研究[J].中国循证儿科杂志,2013,8(3):224-227,4.基金项目
国家自然科学基金:81270797 （）
江苏省杰出青年基金:BK2012001 （）
